FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007958929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443052
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA103772.342162996668563No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA54001.218818558543918No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA52041.1745799590115833No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC46161.0418641604145789No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41320.9326219044265684No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT38040.858589962352049No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23580.5322174372308442No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21750.4909130305246337No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20860.4708250950227062No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17930.404692902864675No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12980.29296786833148253No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12440.28077968274604337No Hit
GAATGCGACAACTATCGCCAGGAATACGATA12120.27355705425096827No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC11850.26746296145824866No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10530.23766961891606406No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9700.21893592625696306No Hit
ATCACTCTGTCTCAAAACGGCTACAACACTG9250.20877910493576377No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9020.20358784070492855No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8340.188239755152894No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8340.188239755152894No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8160.1841770266244143No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8080.18237136950064553No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7400.167023283948611No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7280.16431479826295783No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG7220.16296055542013127No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7170.1618320197177758No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6440.1453553984633858No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6310.14242120563726154No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6240.14084125565396388No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6230.14061554851349278No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6080.13722994140642633No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6020.13587569856359977No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5760.13000731291135126No Hit
CTGGGCTGGAAAGCTGGTATCCTGACCGGTT5670.1279759486471114No Hit
AACGTAACCACCAACCAGGACGGCACCGTCT5430.12255897727580509No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5410.12210756299486292No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA5090.11488493449978783No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG5080.11465922735931673No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG4740.10698518458329948No Hit
CAGGTTAGCTATGGTGCTCGCCCGACCCAGA4530.10224533463340647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)