FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007958930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447592
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT48861.0916191531573396No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC48341.080001429873635No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC40600.9070760871507981No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC33370.7455450499562102No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG27740.61976085363456No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC23430.5234678010330838No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA22510.5029133675311445No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT22170.4953171638456451No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA20140.4499633594881053No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT19250.4300791792525335No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA17480.3905342365368461No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA14710.32864751827557237No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA12370.2763677634989008No Hit
GTACATGTGCGGACGGTGCGGATGGTTATCGAAACCGTGCGGGT12240.27346333267797457No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12110.2705589018570484No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG11320.2529088991760353No Hit
GCGACGTTTCGTGCTCTGTTTGGTAGAGCTTTTGGTGGTTGCTG10100.2256519330104202No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10050.22453484423314088No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT9140.20420382848665752No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7950.17761711558740997No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT7920.17694686232104237No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7690.17180825394555757No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7610.17002091190191068No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA7510.16778673434735206No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG7290.1628715437273231No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7260.16220129046095552No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6720.150136731666339No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT6440.14388103451357487No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6420.14343419900266313No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6360.14209369246992795No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT6300.1407531859371928No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6240.13941267940445762No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA6210.13874242613809004No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG6060.1353911598062521No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5870.13114622245259075No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC5710.12757153836529697No Hit
CACCTGACCATTGGCGTGAGTGGTAACGTTGTAGGCGTTAGTTT5330.11908166365797422No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5320.11885824590251837No Hit
ACCGTGCTGGGAGAACAGGCGACCGGCGCTTTTCGGGTTTGCCT5270.11774115712523905No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT4820.10768735812972528No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4780.10679368710790184No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC4500.10053798995513773No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG4480.10009115444422599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)