FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007958931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447592
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA106242.3735902339630734No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA54491.2174033494789898No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA52441.1716027096105381No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC46881.047382437577079No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40440.9035014030635042No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT37960.8480937997104506No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA24650.5507247671986989No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT21630.4832526050510286No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21270.47520956585461765No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19300.43119626802981287No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13280.2966987792453842No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12670.2830702961625767No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC12030.2687715598134015No Hit
GAATGCGACAACTATCGCCAGGAATACGATA11890.2656437112370194No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10180.22743927505406708No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9820.21939623585765605No Hit
ATCACTCTGTCTCAAAACGGCTACAACACTG9010.2012993976657313No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8910.19906522011117264No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8520.19035192764839406No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8410.1878943323383796No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8200.18320255947380648No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7880.17605319129921893No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7310.1633183792382348No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG7300.16309496148277897No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7290.1628715437273231No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7240.16175445495004379No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG6820.1523709092208976No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6610.14767913635632451No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6580.1470088830899569No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6570.14678546533450107No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6530.14589179431267763No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA6460.14432787002448658No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6370.14231711022538385No Hit
CTGGGCTGGAAAGCTGGTATCCTGACCGGTT5770.12891204489803215No Hit
AACGTAACCACCAACCAGGACGGCACCGTCT5730.12801837387620868No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5710.12757153836529697No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA5310.11863482814706251No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG5010.11193229548338665No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4820.10768735812972528No Hit
CAGGTTAGCTATGGTGCTCGCCCGACCCAGA4490.10031457219968185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)