FastQCFastQC Report
Sun 21 May 2023
EGAF00007958946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007958946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420027
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA21580.5137764953205389No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT18410.4383051565732679No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA18040.4294961990538703No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC14640.34854902184859543No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG14460.344263583055375No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT13410.3192651900949225No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG10950.2606975265875765No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG10860.2585548071909663No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA10140.24141305201808455No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT9530.22689017610772638No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG9330.22212857744859257No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG9020.21474809952693516No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG8920.21236730019736824No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7880.18760698716987242No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG7640.18189306877891182No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG7530.17927418951638824No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG7510.17879802965047484No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG7450.1773695500527347No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6840.16284667414237655No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6800.16189435441054978No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6590.15689467581845928No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA6270.14927611796384518No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG5980.14237179990810114No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5950.14165756010923106No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG5440.12951548352843983No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG5410.12880124372956978No Hit
CAGAGCGAAAGTAATGTTTTTGCCATCACGCTTAACCTTCAGGT5090.12118268587495565No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG4690.11165948855668803No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT4650.11070716882486127No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT4640.11046908889190457No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG4610.1097548490930345No Hit
CGGCAGCTTCTCAGTCGGCAGTTTGGACAGGGTTTCGGAACGCT4360.10380285076911722No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4350.10356477083616053No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)