FastQCFastQC Report
Wed 31 May 2023
EGAF00007959160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523940
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT32930.6285070809634691No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG29280.5588426155666679No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG29140.5561705538802153No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG25970.49566744283696607No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC24700.4714280261098599No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT23760.4534870405008207No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG21380.4080619918311257No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT21270.40596251479177003No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG21070.4021452838111234No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG20160.3847768828491812No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG17870.34106958812077715No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC16900.322556017864641No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG15320.29239989311753256No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG15180.2897278314310799No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA14220.27140512272397604No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG13860.2645341069588121No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC13760.26262549146848874No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT13420.2561361988013895No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA12960.2473565675459022No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG12610.24067641332977058No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12310.2349505668588006No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC11270.2151009657594381No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11140.2126197656220178No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG10890.20784822689620946No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT10850.20708478070008016No Hit
CACCTGACCATTGGCGTGAGTGGTAACGTTGTAGGCGTTAGTTT10320.1969691186013666No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG9380.17902813299232737No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT9180.1752109020116807No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9010.1719662556781311No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG8950.1708210863839371No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA8860.16910333244264608No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC8250.15746077795167385No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG8230.15707905485360918No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG7920.1511623468336069No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG7900.15078062373554224No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA7890.1505897621865099No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC7800.14887200824521893No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7560.14429133106844294No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7550.1441004695194106No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG7510.14333702332328127No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG7330.13990151544069931No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT7220.13780203840134367No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG7010.13379394587166468No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT6960.13283963812650304No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6840.13054929953811503No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG6660.12711379165553308No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6560.12520517616520976No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6220.11871588349811046No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG6200.1183341604000458No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG6140.1171889911058518No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG5980.11413520632133452No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG5800.11069969843875253No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5790.1105088368897202No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT5770.11012711379165553No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5660.10802763675229989No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT5510.1051647135168149No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA5490.10478299041875024No Hit
AACGCTCTGGGTGTGTTTCGGACCAGAGGAAACGCTGGCCAGGG5280.10077489788907128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)