FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007959161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523940
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA54031.0312249494216896No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT33610.6414856662976677No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC32770.6254532961789518No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG31610.6033133564912012No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG31220.5958697560789403No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG31160.5947245867847464No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA26380.5034927663472917No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG23310.4448982707943657No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA22320.4260029774401649No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA22220.42409436194984157No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG21510.41054319196854605No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG21410.4086345764782227No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC19240.36721762033820665No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC18180.34698629614077947No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG17920.34202389586593884No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14960.2855288773523686No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG14910.2845745696072069No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14140.2698782303317174No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG13360.2549910295071955No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG12330.23533228995686528No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC11860.2263617971523457No Hit
AACGTAACCACCAACCAGGACGGCACCGTCT11750.22426232011299005No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG11580.2210176737794404No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC11420.21796388899492308No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11350.21662785815169677No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10360.1977325647974959No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9700.18513570256136197No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9480.1809367484826507No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9270.17692865595297172No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9230.17616520975684238No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA9090.17349314807038974No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG9010.1719662556781311No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8790.1677673015994198No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT8740.16681299385425813No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8550.1631866244226438No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG8440.16108714738328817No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8070.1540252700690919No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7970.15211665457876858No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG7940.15154406993167158No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC7780.14849028514715426No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7670.1463908081077986No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7570.14448219261747527No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7540.14390960797037827No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7520.1435278848723136No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT7480.1427644386761843No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC7440.14200099248005496No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7030.13417566896972935No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6990.13341222277360004No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6980.1332213612245677No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG6800.12978585334198572No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC6620.12635034545940377No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6490.12386914532198344No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6300.12024277589036912No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG6160.11757071420391647No Hit
GGTATGAACACCGCGAACACCGCTGGTACTT6120.11680726800778714No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6090.11623468336069015No Hit
TGCCCGGACTTCAAGCTGCGTCTGATGAAGG5890.11241745238004353No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5860.11184486773294652No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5750.10974539069359088No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA5660.10802763675229989No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC5650.10783677520326755No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5520.10535557506584722No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5490.10478299041875024No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5430.10363782112455625No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA5410.10325609802649158No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5410.10325609802649158No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG5360.10230179028132991No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC5330.10172920563423292No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5300.10115662098713592No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5250.10020231324197428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)