FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007959162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499029
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT31780.6368367369431436No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG27130.5436557795238354No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG27030.5416518879664308No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG25150.5039787266872267No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC23710.4751226882606021No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT21880.4384514727601001No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG21390.42863240412881815No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT20110.40298259219404087No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG19150.3837452332429578No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG18630.3733249971444545No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG16430.32923938288155596No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC16240.3254319889224875No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG14690.29437166978271806No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG14090.28234832043829117No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC13850.2775389807005204No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA13770.2759358674545968No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT13590.27232886265126877No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG13070.2619086265527655No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA12210.24467515915908697No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12020.24086776520001846No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG11270.22583857851948483No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10390.20820433281432543No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA10370.20780355450284455No Hit
CACCTGACCATTGGCGTGAGTGGTAACGTTGTAGGCGTTAGTTT9920.19878604249452436No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT9740.19517903769119632No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG9420.18876658470750196No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT8970.17974907269918183No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA8920.17874712692047956No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG8830.17694362451881554No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8710.17453895464993016No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC8380.16792611251049538No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG8360.16752533419901447No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7930.15890860050217523No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA7740.15510120654310672No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG7650.15329770414144267No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG7610.1524961475184809No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG7540.15109342342829776No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC7510.1504922559610764No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT7410.14848836440367194No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7170.14367902466590118No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG7030.14087357648553492No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG6940.1390700740838709No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG6710.13446112350184056No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6580.13185606447721473No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6440.12905061629684847No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT6410.12844944882962714No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG6350.12724711389518445No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG6200.12424127655907773No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT5710.11442220792779578No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG5710.11442220792779578No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5640.11301948383761265No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG5620.11261870552613175No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5500.11021403565724637No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG5430.10881131156706322No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT5370.10760897663262055No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA5290.10600586338669696No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA5220.10460313929651383No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5180.10380158267355204No Hit
AACGCTCTGGGTGTGTTTCGGACCAGAGGAAACGCTGGCCAGGG5050.1011965236489262No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5020.10059535618170488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)