FastQCFastQC Report
Sun 21 May 2023
EGAF00007959163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499029
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA50731.0165741870712925No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43200.8656811527987353No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT32250.6462550272629446No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG30160.604373693713191No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC29720.5955565708606113No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG28920.5795254384013755No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG28840.5779223251554518No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24460.49015187494113566No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG23500.47091451599005263No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21020.42121800536642157No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA20910.41901372465327663No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG19860.39797286330052967No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG19420.3891557404479499No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC18880.37833472603796575No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC16850.33765572742265476No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG16230.32523159976674704No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG14250.2855545469301384No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14230.28515376861865743No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC13820.2769378132332991No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11890.2382627061753926No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG11310.22664013514244663No Hit
AACGTAACCACCAACCAGGACGGCACCGTCT10930.21902534722430964No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10830.21702145566690512No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA10800.2164202881996838No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG10660.21361484001931752No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC10280.2060000521011805No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9600.19237358951083003No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9140.18315568834676943No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA8850.17734440283029645No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA8800.1763424570515942No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8690.17413817633844927No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8550.171332728158083No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT8210.1645194968629078No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG8140.16311677277272463No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG8020.16071210290383925No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7940.15910898965791567No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7880.15790665472347298No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7780.15590276316606852No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT7390.14808758609219103No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC7380.14788719693645058No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG7320.14668486200200792No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC7170.14367902466590118No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7130.14287746804293938No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7120.14267707888719894No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6880.13786773914942818No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA6880.13786773914942818No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6660.13345917772313834No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6310.12644555727222265No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG6280.12584438980500132No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG6260.12544361149352043No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC6260.12544361149352043No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT6240.12504283318203951No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6150.1232393307803755No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC5950.11923154766556653No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5760.11542415370649803No Hit
GGTATGAACACCGCGAACACCGCTGGTACTT5720.11462259708353623No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5710.11442220792779578No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5490.11001364650150593No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5470.10961286819002503No Hit
TGCCCGGACTTCAAGCTGCGTCTGATGAAGG5400.1082101440998419No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA5350.10720819832113965No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5350.10720819832113965No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5220.10460313929651383No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5060.10139691280466666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)