FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007959170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412102
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG35380.8585253165478449No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT29490.7155995360371947No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT21520.522200814361493No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA15000.3639875564787358No Hit
CAGCGTCGGAATGGAGCGACGGGTCTTGATACCGTTGCCGCTTT14150.3433615949449408No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT12220.2965285293446768No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG10630.2579458483579308No Hit
GTCAGTGCCGAAGGACTGACGCAGCTTGGTGCGAACCTGGTTTG9740.23634925334019247No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA8230.19970783932133307No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT7920.19218542982077252No Hit
AGATTTCATAGCGAATGGCAGGGTCGCAATGTCTGCAATGGTCG7670.18611897054612694No Hit
CAGGGTCGGAATCTGATGACGGGTTTTGCATGCAACACCGTTCT7420.18005251127148134No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT6700.162581108560502No Hit
CACATCTGCGCTCGCAGAGATGCCAGCGAAGTAACCGGTTGCAT6340.15384540720501236No Hit
GTAACCCTGCGCGATTTTAATCTGAGAGACACCGTATTTATCGG6330.15360274883402653No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6310.1531174320920549No Hit
TTTAACGTTAGAGGTGAATACGCCACCGAAGCGTGGACCCAGAC6240.15141882349515412No Hit
GATGCTGGAAATGTTGGAGCGAGATTTAACCAGGGTAACAGAGC6130.14874958141431005No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5870.1424404637686786No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC5690.1380726130909338No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT5380.13055020359037325No Hit
GTTCAGGCCACGGATCTGACCGCGCATTTTTTCGCTAATGGACA5220.12666766965460008No Hit
ATCCTTCAGCTCCAGAACTTCCAGCTGCGCATTTTTCAGACGGT5220.12666766965460008No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG5150.12496906105769932No Hit
GCCAGCGGTGTTCAGAGAACGGCGTTCTTTTGCGACGCTAGAGT5070.12302779408981271No Hit
CGGGGCGACTTCACGTTGAGCAGTCTGTACGTTGCCACGCAGTT4970.12060121037995447No Hit
AACCAGTTCCGGAGTCGGCGGACGCGGCGGGCGCTGCAGGATTT4590.11138019228249317No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA4530.10992424205657823No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC4510.10943892531460658No Hit
CGGCTGACCGTCATCTGCTACATTGGTACAGACGCGTGCGCCGT4510.10943892531460658No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA4420.10725499997573416No Hit
GCTGACAACCAGCTCGTCTTCGCCGTTCGGCGGTGGAGGCGGAA4350.10555639137883338No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA4250.10312980766897514No Hit
ATCCTTCATCAGCAGTTCTTCGGAAAACAGGGCATCATACGGCA4200.10191651581404604No Hit
TTCACCGCTGGTACGAATGATGTTACGTACTTTAATCATTTTAT4130.10021790721714528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)