FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007959172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414069
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG35910.8672467632206227No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT29260.7066455107723593No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT20880.5042637821232693No Hit
CAGCGTCGGAATGGAGCGACGGGTCTTGATACCGTTGCCGCTTT14780.35694534002786976No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA14400.34776812560225473No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT12620.30478012118753156No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG9920.2395735976371088No Hit
GTCAGTGCCGAAGGACTGACGCAGCTTGGTGCGAACCTGGTTTG9680.23377746221040452No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA8730.2108344261463669No Hit
CAGGGTCGGAATCTGATGACGGGTTTTGCATGCAACACCGTTCT7220.17436707408668603No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT7100.17146900637333393No Hit
GTAACCCTGCGCGATTTTAATCTGAGAGACACCGTATTTATCGG6950.16784642173164377No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6830.16494835401829164No Hit
AGATTTCATAGCGAATGGCAGGGTCGCAATGTCTGCAATGGTCG6700.16180878066216017No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT6630.16011824116270476No Hit
CACATCTGCGCTCGCAGAGATGCCAGCGAAGTAACCGGTTGCAT6520.157461679092132No Hit
TTTAACGTTAGAGGTGAATACGCCACCGAAGCGTGGACCCAGAC6410.1548051170215592No Hit
CACAGCCGGCGGACCGGTATCAGACGGAGAGACGGTCGGTGGCG5830.14079778974035728No Hit
GATGCTGGAAATGTTGGAGCGAGATTTAACCAGGGTAACAGAGC5730.13838273331256384No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC5570.13451864302809435No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5440.1313790696719629No Hit
GCCAGCGGTGTTCAGAGAACGGCGTTCTTTTGCGACGCTAGAGT5380.12993003581528684No Hit
ATCCTTCAGCTCCAGAACTTCCAGCTGCGCATTTTTCAGACGGT5070.12244336088912719No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT5030.1214773383180098No Hit
CGGGGCGACTTCACGTTGAGCAGTCTGTACGTTGCCACGCAGTT4990.12051131574689243No Hit
GTTCAGGCCACGGATCTGACCGCGCATTTTTTCGCTAATGGACA4890.11809625931909898No Hit
GCTGACAACCAGCTCGTCTTCGCCGTTCGGCGGTGGAGGCGGAA4860.11737174239076097No Hit
CGGCTGACCGTCATCTGCTACATTGGTACAGACGCGTGCGCCGT4720.11399066339185014No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA4560.11012657310738065No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC4460.10771151667958723No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA4370.10553796589457312No Hit
GTAGCCAGAACCATCGGAACCGGTAACCGGAGCTGCTTTGGAGC4300.10384742639511772No Hit
GTAGAAACCCGGGCCCTTGCCTTTGTGGCCGCAAAAGTCACAAA4190.10119086432454495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)