FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007959175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382759
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT46821.2232240130212484No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG23880.6238912736212604No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19830.5180805676679059No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC17860.4666121502041755No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA17250.4506752290605838No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11680.30515285074942716No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11290.2949636716576227No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10740.2805943165281548No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9910.258909653332776No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9620.2513330842645111No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7560.197513317779595No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7140.1865403556807286No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7070.18471152866425086No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7050.18418900665954296No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA7040.183927745657189No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7000.18288270164777315No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA6970.18209891864071126No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6910.18053135262658748No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6750.1763511765889241No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5920.15466651339354529No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5890.15388273038648342No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC4950.12932419616521101No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4940.12906293516285705No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA4940.12906293516285705No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4890.12775663015108724No Hit
AACCGTGACCCGGTGGAAGCAGCTAACCCGT4610.12044132208517631No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT4340.11338727502161934No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC4270.11155844800514161No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG4220.11025214299337181No Hit
ATTGAAGTTATCGACGTTGAAACCACTGAAG4120.1076395329698322No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4000.10450440094158465No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG3920.10241431292275296No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA3870.10110800791098315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)