FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007959259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374931
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37150.9908489828795166No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA33440.8918974424627465No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT25270.6739906809519618No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22010.5870413489415386No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA17520.4672859806204342No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA16030.42754533500830816No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC15880.4235445988728593No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG14740.39313900424344744No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13170.35126463269241537No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9870.26324843771253914No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9860.26298172197017583No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA9640.2571139756381841No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9290.24777892465546997No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC9010.24031088386929864No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT8370.2232410763580499No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7690.2051044058773481No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7440.19843651231826656No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA7320.19523592340990742No Hit
GATGGTGAACGTAACAACCAGGTTCTGGAAG7100.18936817707791567No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT7030.18750116688137283No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6960.18563415668483002No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6770.18056655757992804No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6710.17896626312574848No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6560.17496552699029955No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6370.16989792788539756No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6050.1613630241297732No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6010.16029616116032017No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC6010.16029616116032017No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC6000.1600294454179569No Hit
CTGGACAGCCTGCGCATGGAGATGGAACGTC5800.15469513057069167No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG5590.14909409998106318No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5580.14882738423869993No Hit
TCCGCGGCCGAAAAAACCAAAGGTAACGCGA5490.14642694255743055No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5480.14616022681506732No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5480.14616022681506732No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA5420.14455993236088774No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5200.138692186028896No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA5170.1378920388018062No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA5090.1357583128629001No Hit
GAACAGGGCCCGGCGGACGATCCGGGTGAGG5040.1344247341510838No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG5010.13362458692399404No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT4790.12775684059200226No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC4740.12642326188018596No Hit
GAACAGGGTCCGGCAGACGATCCGGGTGAAG4660.12428953594127987No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4490.11975536832110442No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4420.11788835812456158No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT4400.11735492663983507No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA4280.11415433773147592No Hit
GGTCAAGGTCAACAGGGTTACTACCCGATCT4280.11415433773147592No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG4070.10855330714184742No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC3960.10561943397585155No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA3870.1032189922945822No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)