FastQCFastQC Report
Wed 31 May 2023
EGAF00007959882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435558
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA32220.7397407463529541No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA31140.7149449671455925No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT22070.5067063399133984No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC21290.4887982771525262No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC15990.36711528659788134No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT15520.3563245308317147No Hit
CGGAACCGGCAGTGGCGGTTCGGTGGAACGGGATGCGATCAGCG15050.3455337750655481No Hit
AATATCAACGGTCTGGACACCCAGGCCCGGTGCCACTTTCTTGA13260.3044370669348284No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG12100.27780456334173637No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG11920.2736719334738427No Hit
CTTCACTTTACGATCCGGTTTCAGGTTACGGTCGACCGGAGGCG11610.26655462647913714No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC11280.2589781383879988No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG11110.25507509906832154No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10860.2493353353629138No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA10030.23027931986095998No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC9520.21857020190192808No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG9250.2123712571000877No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC9130.20961617052149198No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8850.20318763517143526No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG8690.1995141863999743No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG8510.1953815565320807No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG8370.19216728885705234No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA8270.1898713833748892No Hit
GGCCGGCTTTTTCGGTGGCTGAACTGGCGGTTTCGGAGCACCCG7840.17999898980158785No Hit
AACGTTCAGCGCTACGAACTGTGCTTTCGCCATAGCGTCAGAAA7100.16300928923358085No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6980.1602542026549851No Hit
GGAAAAGCCGAAGCGACGCAGCGCACGAGTGTCTGCGGTTACAC6480.14877467524416954No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6420.1473971319548717No Hit
TGGGTCCGGCTTGCGGGTAGCTGCGCTAGTCGGGGCGACAGCCG6230.14303491153876177No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA5870.1347696518029746No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA5770.13247374632081146No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT5690.13063702193508098No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT5590.12834111645291787No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC5310.12191258110286116No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT5300.12168299055464483No Hit
GTCAGTGCCGAAGGACTGACGCAGCTTGGTGCGAACCTGGTTTG5260.1207646283617796No Hit
CGCCAAATTTAAATGGCCGCCTCATTTACGGCTGGCAGCGGAGG5260.1207646283617796No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG4930.11318814027064134No Hit
TTCGTCACCGGTGCTCGGCAGCTTACGCTTGTTGTTAGTGGATT4900.1124993686259924No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT4680.10744837656523355No Hit
AGATGGCGCGTTGGAAGTAACTTTGTTGTTGGTGTCATCGGCGG4620.1060708332759357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)