FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007959883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435558
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT76301.7517758828904533No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA48631.1164988359759205No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24610.5650223391603415No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21410.4915533637311219No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA16080.36918160153182816No Hit
CGTCGCGGTCAGAAAGCCAAATCTAACCCGA15440.35448780644598427No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG15220.3494368143852254No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC13500.3099472400920199No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA13310.30558501967590995No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG12440.28561064198109093No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG12080.2773453822453037No Hit
CCGGGTACCTTCGACTTCTCCTCTTTTGGTA11930.27390152402205903No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG11250.2582893667433499No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG10880.2497945164593464No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10830.24864656371826485No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC9680.22224365067338908No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG8910.20456517846073313No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8750.20089172968927216No Hit
ATGTCCAACATGGACATCGATGGTATTAACA8740.20066213914105585No Hit
GTAGGCTCCGTGTTCAAAGAAATCATTAACC8170.1875754778927261No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8160.1873458873445098No Hit
GAAACCACTATCGCAGATAATAGCAAACGCT7560.17357045445153113No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA7090.16277969868536452No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7010.16094297429963403No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6570.15084099017811634No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA6460.14831549414773693No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG6440.1478563130513043No Hit
GAAGAGCTGTCCAACGAAGTTCTGCCACCGC6380.14647876976200644No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6210.14257573044232913No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC5820.13362169906189303No Hit
AACACTTCTAGCGGTCAGACCCAATCTACCA5450.12512684877788952No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5240.12030544726534698No Hit
GCGGAGCAGCACACGCCGATGAAGGCGCACG5130.11777995123496757No Hit
TCCCAGGAAATCACCTTCGTTTACGAGAAAA5070.1164024079456697No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT5060.11617281739745339No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG4740.10882591985453144No Hit
CTGCGTGTCTGCGCTGCGCTGTCCATCCCGA4660.10698919546880094No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG4380.10056066011874423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)