FastQCFastQC Report
Wed 8 May 2024
EGAF00007959884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412449
Total Bases18.1 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA30330.7353636449597405No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA28690.6956011531122636No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT20560.4984858734049543No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC20260.49121224684748904No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC15460.37483422192804444No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT14970.3629539652175178No Hit
CGGAACCGGCAGTGGCGGTTCGGTGGAACGGGATGCGATCAGCG14470.350831254288409No Hit
AATATCAACGGTCTGGACACCCAGGCCCGGTGCCACTTTCTTGA12290.29797623463749456No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG11730.28439879839689275No Hit
CTTCACTTTACGATCCGGTTTCAGGTTACGGTCGACCGGAGGCG11400.2763978091836809No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG11230.2722760874677839No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC10180.2468183945166554No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG9680.23469568358754658No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC9350.22669469437433476No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8870.2150568918823903No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC8850.21457198344522593No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG8730.21166253282223985No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC8620.20899553641783591No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8430.20438890626477457No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG8170.19808509658163798No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG8100.19638791705156272No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG8080.19590300861439838No Hit
GGCCGGCTTTTTCGGTGGCTGAACTGGCGGTTTCGGAGCACCCG7580.18378029768528958No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA7490.18159820971805No Hit
AACGTTCAGCGCTACGAACTGTGCTTTCGCCATAGCGTCAGAAA7240.17553685425349558No Hit
GGAAAAGCCGAAGCGACGCAGCGCACGAGTGTCTGCGGTTACAC6640.160989601138565No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6490.15735278785983237No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6290.15250370348818884No Hit
TGGGTCCGGCTTGCGGGTAGCTGCGCTAGTCGGGGCGACAGCCG6130.14862443599087402No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT5650.13698663349892956No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA5640.1367441792803474No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT5320.12898564428571774No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT5280.12801582741138903No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA5220.126561102099896No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG5200.12607619366273162No Hit
CGCCAAATTTAAATGGCCGCCTCATTTACGGCTGGCAGCGGAGG5170.1253488310069851No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC5100.12365165147690986No Hit
TTCGTCACCGGTGCTCGGCAGCTTACGCTTGTTGTTAGTGGATT4890.11856011288668418No Hit
GTCAGTGCCGAAGGACTGACGCAGCTTGGTGCGAACCTGGTTTG4660.11298366585929412No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT4320.10474022242750014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)