FastQCFastQC Report
Wed 8 May 2024
EGAF00007959886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439985
Total Bases19.3 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA32020.7277520824573565No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA30720.6982056206461584No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT22180.5041080945941339No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC21740.4941077536734207No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC17350.3943316249417594No Hit
CGGAACCGGCAGTGGCGGTTCGGTGGAACGGGATGCGATCAGCG15900.3613759559985No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT15020.3413752741570735No Hit
AATATCAACGGTCTGGACACCCAGGCCCGGTGCCACTTTCTTGA12950.2943282157346273No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG12660.2877370819459754No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12330.28023682625544055No Hit
CTTCACTTTACGATCCGGTTTCAGGTTACGGTCGACCGGAGGCG11800.26819096105549056No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC10930.24841755968953486No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC10930.24841755968953486No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC10320.23455345068581884No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG10130.23023512165187449No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG9540.21682557359909996No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC9140.2077343545802698No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC9090.20659795220291602No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9080.20637067172744528No Hit
GGCCGGCTTTTTCGGTGGCTGAACTGGCGGTTTCGGAGCACCCG8820.2004613793652056No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG8810.20023409888973487No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG8450.19205200177278772No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG8410.1911428798709047No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA8020.18227894132754524No Hit
AACGTTCAGCGCTACGAACTGTGCTTTCGCCATAGCGTCAGAAA6800.1545507233201132No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6780.15409616236917167No Hit
GGAAAAGCCGAAGCGACGCAGCGCACGAGTGTCTGCGGTTACAC6640.1509142357125811No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6510.1479595895314613No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA6230.14159573621828017No Hit
TGGGTCCGGCTTGCGGGTAGCTGCGCTAGTCGGGGCGACAGCCG6230.14159573621828017No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT6080.13818652908621884No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT6010.13659556575792356No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA5990.13614100480698207No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC5770.13114083434662546No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT5690.1293225905428594No Hit
GTCAGTGCCGAAGGACTGACGCAGCTTGGTGCGAACCTGGTTTG5490.12477698103344431No Hit
CGCCAAATTTAAATGGCCGCCTCATTTACGGCTGGCAGCGGAGG5140.11682216439196792No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG4950.11250383535802357No Hit
TTCGTCACCGGTGCTCGGCAGCTTACGCTTGTTGTTAGTGGATT4650.10568542109390092No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA4530.10295805538825187No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT4440.10091253110901509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)