FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007959889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415866
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT70151.6868414345005363No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA44551.0712585303920013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35590.855804513954014No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA22550.542241972173729No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21100.5073749717457066No Hit
CGTCGCGGTCAGAAAGCCAAATCTAACCCGA15150.36430003895485563No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA14730.354200631934325No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA13690.3291925764549158No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG13510.3248642591604026No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC13060.3140434659241198No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG11680.2808596999995191No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG11370.2734053757700798No Hit
CCGGGTACCTTCGACTTCTCCTCTTTTGGTA11010.2647487411810535No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG10050.24166438227698345No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG9450.22723665796193968No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9330.22435111309893088No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC8890.21377078193456547No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG8620.20727830599279576No Hit
ATGTCCAACATGGACATCGATGGTATTAACA8280.1991025955476043No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA8240.1981407472599347No Hit
GTAGGCTCCGTGTTCAAAGAAATCATTAACC7900.18996503681474322No Hit
GAAACCACTATCGCAGATAATAGCAAACGCT7160.17217084349285586No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7080.1702471469175167No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA6890.16567836755108617No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG6400.15389572602713372No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6130.14740325008536403No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA6040.14523909143810748No Hit
GAAGAGCTGTCCAACGAAGTTCTGCCACCGC6030.14499862936619007No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC5830.14018938792784214No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5810.13970846378400736No Hit
TCCCAGGAAATCACCTTCGTTTACGAGAAAA5250.12624258775663313No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5230.12576166361279834No Hit
AACACTTCTAGCGGTCAGACCCAATCTACCA5150.12383796703745917No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT5050.12143334631828523No Hit
GCGGAGCAGCACACGCCGATGAAGGCGCACG4660.11205532551350676No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG4270.1026773047087283No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG4270.1026773047087283No Hit
CTGCGTGTCTGCGCTGCGCTGTCCATCCCGA4240.10195591849297611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)