FastQCFastQC Report
Wed 8 May 2024
EGAF00007959916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421671
Total Bases18.5 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCCGTCGATGTTACGGACCGGAATCAGCAGTGCACCATCAA28360.6725622582534725No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT24850.5893220069675174No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG19700.46718887473883663No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT13800.3272693640302511No Hit
GGTATCGGAAGAGTCGTCATCCTCTTCTTCGGAGTCTTCTACCG12050.2857678142438062No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG12030.2852935108176754No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG10720.2542266364061081No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT9460.22434552055986776No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA9370.22221115514227918No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8360.19825883212267384No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT7700.18260681906035747No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7340.1740693573900031No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6860.16268607516286396No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6840.16221177173673312No Hit
AGGCGGGATGGCGATGAAGGACGCGTGCGGGCGCGGACGACGCG6490.15391146177944415No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG6410.15201424807492098No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6260.14845697237893998No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6230.14774551723974377No Hit
AGTGGTGTGACCCAGGCTTACAACGGAGCCGGTATCGGAAGAAT5950.1411052692739126No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5890.1396823589955202No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA5870.13920805556938942No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC5610.13304211102968902No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5390.12782477334225023No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5310.12592755963772706No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5270.12497895278546543No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT5200.12331889079400765No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT5150.12213313222868066No Hit
TACGGAAGCAGATGCGCTCGGCTGACGGGCGGAAGACGCAGAAG4720.11193560856686847No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA4650.1102755465754107No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG4630.1098012431492799No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT4620.1095640914362145No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG4590.1088526362970183No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG4490.1064811191663643No Hit
AACAGCGGTAGACGGAGCGGAAGAGGCCAGAGCGCCAGTACCCG4370.1036352986095795No Hit
ACGGATGATGGTGGAGGCTTCGAAACGCACTGCTTCAACATCCG4250.10078947805279473No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)