FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007959920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428739
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCCGTCGATGTTACGGACCGGAATCAGCAGTGCACCATCAA29620.6908632058198577No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT25510.5950006880643002No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG20160.4702161454871146No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14050.32770520059989877No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG12090.28198974201087373No Hit
GGTATCGGAAGAGTCGTCATCCTCTTCTTCGGAGTCTTCTACCG11600.2705608773636175No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG10890.25400068573187884No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT10100.23557455701487384No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA9360.21831463897616032No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8620.2010547209374468No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT7730.1802961708638589No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7550.17609781242200964No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6910.16117031573987903No Hit
AGGCGGGATGGCGATGAAGGACGCGTGCGGGCGCGGACGACGCG6670.15557250448408005No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6350.14810875614301475No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6300.1469425454647233No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA6120.14274418702287406No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG6100.14227770275155746No Hit
AGTGGTGTGACCCAGGCTTACAACGGAGCCGGTATCGGAAGAAT6080.1418112184802409No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC5890.13737961790273337No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5810.13551368081746704No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT5730.1336477437322007No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT5620.1310820802399595No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5560.12968262742600975No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5430.12665047966245196No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG5370.12525102684850223No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5010.11685430996480377No Hit
ACGGATGATGGTGGAGGCTTCGAAACGCACTGCTTCAACATCCG4800.11195622511597965No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG4790.11172298298032135No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG4710.10985704589505504No Hit
TACGGAAGCAGATGCGCTCGGCTGACGGGCGGAAGACGCAGAAG4680.10915731948808016No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA4440.10355950823228117No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG4340.10122708687569826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)