FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007959922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462341
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT36060.7799438077090286No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA31500.6813153062350084No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG28500.6164281342126267No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG23690.5123923684034078No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22930.4959542848244045No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG22420.4849234655805996No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG21610.46740392913455653No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG20660.44685632466080233No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG20120.43517663369677356No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT19670.42544355789341637No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG19160.4144127386496115No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA18900.408789183741005No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG16590.35882606128377104No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG16560.35817718956354727No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG16380.35428395924220435No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA15630.3380621662366089No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG15280.33049199616733105No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG13790.29826470072954814No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT13610.29437147040820516No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG13600.2941551798347973No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG13270.28701759091233525No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12800.2768519339621621No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12800.2768519339621621No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12690.2744727376546748No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA12560.2716609602003716No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT12430.26884918274606834No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT12150.26279304669064607No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG12120.26214417497042225No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC11820.25565545776818405No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG11140.2409476987764442No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG11070.23943366476258865No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10850.23467527214761397No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC10650.23034946067945522No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10460.22623993978470436No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT10400.22494219634425672No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA10000.21629057340793917No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG9970.21564170168771538No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG9870.21347879595363597No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG9760.21109959964614863No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9750.2108833090727407No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG9220.1994199086821199No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT9150.19790587466826434No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT8770.18968683287876265No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG8520.18427956854356414No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG8500.1838469873967483No Hit
GAAGGTGCCGGTGCCAGCCACGCTCGGTGCAAAAATGGCGTTGG8350.1806026287956292No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT8260.17865601363495776No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA8190.17714197962110217No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8190.17714197962110217No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8120.1756279456072466No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG7910.17108584356567988No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT7850.16978810012523224No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7770.16805777553796875No Hit
CGCTTTGGTAACCTGCGGGCTCACCAGGCTGAATGCTGCGTGAC7540.16308309234958615No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7070.152917435399413No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC7020.1518359825323733No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6980.15097082023874153No Hit
AGATTCAACATCCGTTTCGCTACCGTTGAAGTGCATGTAGGTGG6850.14815904278443834No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA6730.14556355590354306No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT6460.1397237104215287No Hit
ACCGTGGTCGCCGTTGTCGCCATCGTCACCATCGTCGCCGTCAT6000.1297743440447635No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC5840.12631369487023647No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5820.1258811137234206No Hit
TGGCTGTTCGTTATCTACCTCGATGATTGCAACGCACGCACCAC5790.12523224200319677No Hit
CAGATTTTCTTCGTGTGCGTCCACGTTGTTATGGTTGTAGGAGT5750.12436707970956502No Hit
TTTCACGTGTTCATACTTAGAAACATCGAAACCGTCCTGTTTGG5670.1226367551223015No Hit
ATCCTTCATCAGCAGTTCTTCGGAAAACAGGGCATCATACGGCA5530.11960868709459035No Hit
CAGGAAGTTTTCTACAGAGGATTCAGTGCGAGAGTGGTAGTTTT5530.11960868709459035No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5490.11874352480095861No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG5370.11614803792006333No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5320.11506658505302363No Hit
TTCATCACCATCACCGCCTTCGTCACCGTCATCGCCGTCATCGC5320.11506658505302363No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5270.11398513218598394No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT5250.11355255103916806No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG5090.11009190186464103No Hit
TTCTTCCAGTGCGCTCAGTTTAGAGTATGGAATGAAATGATAGT5060.10944303014441721No Hit
GATTTCGTTAACGATATCGCTGTCCTTCAGCTGATAGTTCGGGT5020.10857786785078546No Hit
ACGAACCTGGATGCGAATGGTACGGTTATCAACTTTAACCAGAT4710.10187286007513935No Hit
CTCCTGACCTTCTTCACCTTCATCACCATCACCGCCTTCATCAC4700.1016565695017314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)