FastQCFastQC Report
Wed 8 May 2024
EGAF00007959923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462341
Total Bases14.3 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT36420.7877302683517144No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC33140.7167869602739104No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG32740.7081353373375928No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG32070.6936438689192609No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG27440.593501333431385No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC26080.5640858154479054No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC25320.5476477318689019No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG25150.543970792120967No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG24260.5247209310876604No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA22880.4948728319573648No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG20910.45226358899600083No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20730.44837035867465785No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20420.44166535089901177No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC20240.43777212057766884No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG19760.4273901730540878No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA16990.36747768422008864No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG16830.3640170350455616No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG16660.3603400952976267No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT15860.34303684942499146No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC15600.3374132945163851No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14350.31037697284039273No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14280.3088629388265371No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC13410.2900456589400464No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13400.2898293683666385No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA13280.2872338814857432No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13240.2863687191921115No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG12870.2783659679760177No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12850.2779333868292018No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG12670.27404015650785896No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG12550.2714446696269636No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA11970.25889981636930315No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA11940.2582509446490794No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG11530.24938303113935384No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT11320.24484092909778715No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG11280.2439757668041554No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG10860.2348915627210219No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG10520.22753768322515197No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10410.22515848691766466No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10330.22342816233040116No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG10300.22277929061017732No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9130.19747329352144846No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG9130.19747329352144846No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG9120.1972570029480405No Hit
TCTGCGGACGGCGTAAACTGGACCACTCTGT9100.19682442180122464No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG9070.19617555008100085No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9050.19574296893418494No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8890.19228231975965793No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC8880.19206602918624996No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC8540.18471214969038005No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8230.17800714191473394No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC8090.17497907388702277No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG8090.17497907388702277No Hit
AACATCTTTACCCCGATCAAGAAACCGGGCA7910.17108584356567988No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7670.16589486980388934No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7510.1624342206293623No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7190.15551292228030825No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7180.15529663170690033No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7160.15486405056008443No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC6980.15097082023874153No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6750.14599613705035894No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC6690.1446983936099113No Hit
TCCCAGTCTTCCCAGAAATCCGAGAACCTGA6460.1397237104215287No Hit
CTGCACGACGACGCTAACTTTCTGGAACATG6400.13842596698108106No Hit
CGCGATAACAACATTGAACGTGACAACAATA6070.13128837805861907No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC6060.13107208748521113No Hit
TGGATCGAAGTATCCGGCCAGGAGCTGCCGA5990.12955805347135554No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG5840.12631369487023647No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5820.1258811137234206No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA5590.120906430535038No Hit
CAGATCGTGATCAAAATTACCGATCAGGGTT5550.12004126824140623No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5520.11939239652118243No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC5510.11917610594777447No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5490.11874352480095861No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5400.11679690964028713No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5380.11636432849347128No Hit
TACTATAACGGTAAAGTTCCGTATGATGCAC5320.11506658505302363No Hit
GGTCCAGCTCACTCTAAAGAAGTGCCGGCGC5300.11463400390620776No Hit
GTTGACTCCCTGCACGATGACGGCGATGACG5240.11333626046576013No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5180.11203851702531248No Hit
AAAGAGCTGTACGATTCCCCGTCTGCGCAGC5050.10922673957100928No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG5010.10836157727737751No Hit
GCCCAGTCCGCGACCCCGTCTGTTTCTTCCT4900.1059823809698902No Hit
GAAAAACCGGCCGAACAGCCGCAGCCGGCAC4790.10360318466240287No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG4670.10100769778150759No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)