FastQCFastQC Report
Wed 8 May 2024
EGAF00007959924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436163
Total Bases19.1 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT33370.7650809445092774No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA28940.6635134112705572No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG26640.6107808319366842No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG22630.5188427262284971No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC21380.49018371572095754No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG20600.4723004931642528No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG20320.46588087481056395No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG19800.4539587264394275No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG18600.42644607635218945No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG18060.4140653838129323No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT17840.4090213979636053No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA17290.39641143334028794No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG15800.36224989281530073No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG15690.35972789989063725No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG14980.3434495819223547No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA14480.33198597771933885No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG14440.33106888938309764No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG13200.3026391509596183No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG12950.2969073488581104No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG12840.2943853559334469No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT12780.29300972342908504No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12460.28567301673915485No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC12180.279253398385466No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT12130.2781070379651644No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG11940.2737508683680184No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA11760.26962397085493267No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA11730.2689361546027517No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11720.2687068825186914No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT11030.25288710871852954No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10600.24302840910393592No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG10470.2400478720111518No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG10450.23958932784303114No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA10120.23202334906904068No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG9990.2290428119762566No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC9740.22331100987474867No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9660.22147683320226613No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG9380.21505721484857726No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT9350.2143693985963963No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG9010.2065741477383455No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT8770.20107161772089793No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG8590.1969447202078122No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT8530.1955690877034503No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG8370.19190073435848526No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG8210.18823238101352016No Hit
GAAGGTGCCGGTGCCAGCCACGCTCGGTGCAAAAATGGCGTTGG8190.18777383684539956No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8050.1845640276685551No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG8000.18341766724825354No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG7930.18181276265983132No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7590.17401751180178052No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT7560.17332969554959957No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT7310.16759789344809164No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA7200.16507590052342816No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7160.1641588121871869No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7130.16347099593500594No Hit
CGCTTTGGTAACCTGCGGGCTCACCAGGCTGAATGCTGCGTGAC7130.16347099593500594No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7050.1616368192625234No Hit
AGATTCAACATCCGTTTCGCTACCGTTGAAGTGCATGTAGGTGG6940.15911482633785992No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6620.15177811964792978No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6520.1494853988073266No Hit
ACCGTGGTCGCCGTTGTCGCCATCGTCACCATCGTCGCCGTCAT5860.1343534412593457No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5830.13366562500716475No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG5810.13320708083904412No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA5740.1316021762506219No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC5650.12953872749407905No Hit
CAGATTTTCTTCGTGTGCGTCCACGTTGTTATGGTTGTAGGAGT5500.1260996462331743No Hit
CAGGAAGTTTTCTACAGAGGATTCAGTGCGAGAGTGGTAGTTTT5400.12380692539257113No Hit
TGGCTGTTCGTTATCTACCTCGATGATTGCAACGCACGCACCAC5280.12105566038384731No Hit
TTCATCACCATCACCGCCTTCGTCACCGTCATCGCCGTCATCGC5230.11990929996354574No Hit
ATCCTTCATCAGCAGTTCTTCGGAAAACAGGGCATCATACGGCA5200.11922148371136479No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5080.11647021870264099No Hit
TTTCACGTGTTCATACTTAGAAACATCGAAACCGTCCTGTTTGG5070.11624094661858067No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT5000.11463604203015845No Hit
TTCTTCCAGTGCGCTCAGTTTAGAGTATGGAATGAAATGATAGT5000.11463604203015845No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG4980.11417749786203782No Hit
GATTTCGTTAACGATATCGCTGTCCTTCAGCTGATAGTTCGGGT4970.1139482257779775No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT4820.11050914451707275No Hit
GAACTGGCCGTTGATTTCACCGTAGTCGTGAATTGGAACGATGT4370.10019190073435849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)