FastQCFastQC Report
Wed 8 May 2024
EGAF00007959925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436163
Total Bases13.5 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41330.9475815234212898No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT33170.7604955028280711No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC31130.7137239976797666No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG29760.6823137221635031No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG29170.6687866692039444No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC24670.5656142313768018No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC24520.562175150115897No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG24340.5580482526028113No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG23620.5415406625504685No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG23190.5316819629358749No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA21350.4894958994687766No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG18860.4324071505377577No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC18710.4289680692768529No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG18270.41888009757819894No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18050.41383611172887197No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17860.409479942131726No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG15750.36110353239499915No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG15650.3588108115543959No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA15520.35583027446161186No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT14520.3329030660555801No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC14370.32946398479467537No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13360.3063075043045834No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12990.29782443719435164No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG12890.29553171635374853No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12600.2888828259159993No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12590.28865355383193897No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC12300.2820046633941898No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG12260.2810875750579485No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA12180.279253398385466No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12110.27764849379704376No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12110.27764849379704376No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG11860.27191669169553584No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA10820.2480723949532629No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT10680.24486258577641845No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG10410.2386722395067899No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10170.23316970948934226No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9990.2290428119762566No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG9820.2251451865472312No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG9420.2159743031848185No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG9050.2074912360745868No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG9020.20680341982240585No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG8850.20290579439338047No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8830.20244725022525983No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8710.19969598521653603No Hit
TCTGCGGACGGCGTAAACTGGACCACTCTGT8710.19969598521653603No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG8300.19029582977006304No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC8290.19006655768600272No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG8150.1868567485091583No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC7910.18135421849171066No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7720.17699804889456466No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC7640.17516387222208213No Hit
AACATCTTTACCCCGATCAAGAAACCGGGCA7320.167827165532152No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7210.16530517260748848No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7160.1641588121871869No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7080.16232463551470436No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG7020.16094900301034246No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC7010.16071973092628214No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6830.15659283341319644No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6740.15452938465665358No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6420.14719267796672345No Hit
TGGATCGAAGTATCCGGCCAGGAGCTGCCGA6020.13802179460431077No Hit
TCCCAGTCTTCCCAGAAATCCGAGAACCTGA5880.13481198542746634No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5830.13366562500716475No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA5760.13206072041874253No Hit
CGCGATAACAACATTGAACGTGACAACAATA5750.13183144833468222No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC5620.1288509112418981No Hit
CTGCACGACGACGCTAACTTTCTGGAACATG5620.1288509112418981No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC5600.12839236707377746No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG5500.1260996462331743No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5480.12564110206505366No Hit
CAGATCGTGATCAAAATTACCGATCAGGGTT5200.11922148371136479No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC5190.11899221162730447No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5150.1180751232910632No Hit
GGTCCAGCTCACTCTAAAGAAGTGCCGGCGC5090.1166994907867013No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5030.11532385828233939No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4990.11440676994609812No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4820.11050914451707275No Hit
AAAGAGCTGTACGATTCCCCGTCTGCGCAGC4780.10959205618083147No Hit
ACCCGTAAACATCGTGTGGTTGCTCTGAAAC4610.1056944307518061No Hit
GCCCAGTCCGCGACCCCGTCTGTTTCTTCCT4540.10408952616338388No Hit
GTTGACTCCCTGCACGATGACGGCGATGACG4480.10271389365902198No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG4440.10179680532278071No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA4430.10156753323872039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)