FastQCFastQC Report
Wed 8 May 2024
EGAF00007959926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466777
Total Bases20.5 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT36050.772317402099932No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA29870.6399201331685151No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG28890.6189250969949248No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG23930.5126645057489979No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG22640.4850281826225371No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC22450.48095771642561647No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG21170.4535356283621515No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG21050.45096480760620167No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG21020.45032210241721427No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG20080.4301840064956071No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT19600.4199007234718078No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA19300.4134736715819331No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG17250.36955548366779No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG16740.3586294954550031No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG16300.349203152683187No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG16140.34577539167525395No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA15440.33077893726554647No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG14250.3052849647690439No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG13580.29093121554832396No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT13550.2902885103593365No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG13390.2868607493514033No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12920.2767917013905998No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12790.2740066455716541No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA12760.27336394038266665No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12650.27100735468971265No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG12510.2680080638077712No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT12340.26436606773684224No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC12110.25943866128793835No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT11580.2480842029491599No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG11580.2480842029491599No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT11550.24744149776017244No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG10970.23501586410641487No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10480.22451834601961967No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA10430.22344717070464054No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC10390.22259023045265725No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG10250.2195909395707158No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA10240.21937670450771996No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG9770.20930765654691644No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG9660.2069510708539624No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT9300.19923860858611286No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT9010.19302579175923407No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT8990.19259732163324242No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG8910.19088344112927585No Hit
GAAGGTGCCGGTGCCAGCCACGCTCGGTGCAAAAATGGCGTTGG8830.1891695606253093No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG8750.18745568012134273No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG8570.18359944898741798No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG8480.1816713334204556No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8400.179957452916489No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT8230.17631545684556008No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8160.17481581140458935No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT7970.17074534520766876No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7830.16774605432572726No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA7640.1636755881288067No Hit
CGCTTTGGTAACCTGCGGGCTCACCAGGCTGAATGCTGCGTGAC7460.15981935699488192No Hit
AGATTCAACATCCGTTTCGCTACCGTTGAAGTGCATGTAGGTGG7370.15789124142791955No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7300.1563915959869488No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC7170.15360654016800313No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7080.15167842460104075No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC6820.14610831296314944No Hit
CAGATTTTCTTCGTGTGCGTCCACGTTGTTATGGTTGTAGGAGT6660.14268055195521628No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6650.14246631689222047No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA6220.13325420918340022No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5960.12768409754550888No Hit
TTCATCACCATCACCGCCTTCGTCACCGTCATCGCCGTCATCGC5740.12297092615960084No Hit
ATCCTTCATCAGCAGTTCTTCGGAAAACAGGGCATCATACGGCA5730.12275669109660503No Hit
ACCGTGGTCGCCGTTGTCGCCATCGTCACCATCGTCGCCGTCAT5720.12254245603360919No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG5700.12211398590761756No Hit
TGGCTGTTCGTTATCTACCTCGATGATTGCAACGCACGCACCAC5570.11932893008867189No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5560.11911469502567608No Hit
CAGGAAGTTTTCTACAGAGGATTCAGTGCGAGAGTGGTAGTTTT5500.11782928464770116No Hit
TTTCACGTGTTCATACTTAGAAACATCGAAACCGTCCTGTTTGG5470.1171865794587137No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG5280.1131161132617931No Hit
ACGAACCTGGATGCGAATGGTACGGTTATCAACTTTAACCAGAT5270.1129018781987973No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5270.1129018781987973No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT5180.1109737626318349No Hit
GATTTCGTTAACGATATCGCTGTCCTTCAGCTGATAGTTCGGGT5090.10904564706487252No Hit
TTCTTCCAGTGCGCTCAGTTTAGAGTATGGAATGAAATGATAGT4940.10583212111993522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)