FastQCFastQC Report
Wed 8 May 2024
EGAF00007959927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466777
Total Bases14.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT36470.7813152747457565No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC33050.708046883201186No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG32250.6909080781615203No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG31070.6656283407280136No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG27760.5947165348763971No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC26130.5597962196080785No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG25560.5475848210173166No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC25110.5379442431825047No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA24760.5304460159776511No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG24340.5214481433318265No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG21580.46231926594498013No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20970.4492509271022351No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC20730.4441092855903354No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20420.437467998637465No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG20400.4370395285114734No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG17330.37126936417175654No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA17250.36955548366779No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG17060.3654850174708694No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT15540.3329212878955047No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC15090.3232807100606928No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14310.3065703751470188No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14240.30507072970604804No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13630.29200239086330304No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13480.28878886491836575No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG13140.2815048727765078No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG13040.2793625221465496No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA13030.2791482870835538No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC12900.27636323126460816No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12830.2748635858236374No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG12660.2712215897527085No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA12270.2628664222958715No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT11990.25686784053198847No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG11330.24272832637426436No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA11210.24015750561831453No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11060.23694397967337724No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG10990.2354443342324065No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG10610.2273034018385653No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10420.2232329356416447No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG10270.2200194096967074No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG9830.21059306692489135No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG9470.20288060465704177No Hit
TCTGCGGACGGCGTAAACTGGACCACTCTGT9390.20116672415307524No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC9110.19516814238919228No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG9100.19495390732619647No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9000.19281155669623823No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8770.18788415024733437No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC8710.18659873986935946No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG8580.18381368405041382No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC8490.18188556848345142No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8170.17503004646758516No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8170.17503004646758516No Hit
AACATCTTTACCCCGATCAAGAAACCGGGCA7860.16838875951471474No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG7760.16624640888475653No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7730.16560370369576907No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7690.16474676344378578No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7640.1636755881288067No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7550.1617474725618443No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7410.15874818167990284No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC7250.1553204206719697No Hit
CGCGATAACAACATTGAACGTGACAACAATA7000.1499645440970742No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6700.14353749220719958No Hit
TCCCAGTCTTCCCAGAAATCCGAGAACCTGA6540.14010973119926645No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC6140.13154032867943366No Hit
TGGATCGAAGTATCCGGCCAGGAGCTGCCGA6130.13132609361643782No Hit
CTGCACGACGACGCTAACTTTCTGGAACATG6090.13046915336445453No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC6030.1291837429864796No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5980.1281125676715005No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC5930.12704139235652143No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5880.12597021704154232No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG5860.12554174691555067No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA5790.12404210147457995No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5630.1206143404666468No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5590.11975740021466354No Hit
CAGATCGTGATCAAAATTACCGATCAGGGTT5540.11868622489968443No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5430.11632963920673041No Hit
GTTGACTCCCTGCACGATGACGGCGATGACG5310.11375881845078056No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5290.11333034832478892No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA5280.1131161132617931No Hit
GGTCCAGCTCACTCTAAAGAAGTGCCGGCGC5190.11118799769483073No Hit
TACTATAACGGTAAAGTTCCGTATGATGCAC5070.10861717693888089No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG4940.10583212111993522No Hit
AAAGAGCTGTACGATTCCCCGTCTGCGCAGC4930.1056178860569394No Hit
GCCCAGTCCGCGACCCCGTCTGTTTCTTCCT4750.10176165492301462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)