FastQCFastQC Report
Wed 8 May 2024
EGAF00007959928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440589
Total Bases19.3 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT33060.7503591782818No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA29400.6672885614484247No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG27120.615539652601404No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG23590.5354196314479027No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG21370.485032535991593No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC21190.4809470958194599No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG21100.47890437573339323No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG20360.46210867724795673No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG18600.4221621511204319No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG18430.4183036798467506No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA17940.4071822038226102No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT17510.39742254118918086No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG16190.3674626465935373No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG15720.3567951083663006No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG14730.3343251874195679No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA14160.32138796020781274No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG13980.31730252003567955No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG13070.29664835027656156No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA13010.2952865368858505No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG13010.2952865368858505No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG12900.2927898790028802No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC12430.2821223407756435No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT12350.28030658958802873No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12300.27917174509576953No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA12240.27780993170505847No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12040.27327055373602155No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT11660.26464573559485144No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG11450.25987938872736266No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT11270.2557939485552295No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG11080.2514815394846444No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10650.24172187685121507No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG10570.23990612566360034No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA9990.22674192955339328No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC9700.22015983149828977No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT9460.21471257793544551No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG9420.21380470234163815No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9420.21380470234163815No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT9280.2106271377633123No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG9260.21017319996640862No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT9080.2060877597942754No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG8950.2031371641144014No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG8920.20245625741904588No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG8760.19882475504381636No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG8700.19746294165310527No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT8280.1879302479181278No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8100.18384480774599457No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT8020.1820290565583798No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT7880.17885149198005398No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG7870.17862452308160215No Hit
GAAGGTGCCGGTGCCAGCCACGCTCGGTGCAAAAATGGCGTTGG7650.17363120731566153No Hit
CGCTTTGGTAACCTGCGGGCTCACCAGGCTGAATGCTGCGTGAC7410.16818395375281725No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG7330.1663682025652025No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA7250.16455245137758773No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7000.1588782289162916No Hit
AGATTCAACATCCGTTTCGCTACCGTTGAAGTGCATGTAGGTGG6950.15774338442403238No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT6660.15116128636892887No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6620.1502534107751215No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6270.14230949932930692No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6150.13958587254788477No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA6120.13890496585252923No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC5980.13572740127420338No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG5780.1311880233051665No Hit
CAGATTTTCTTCGTGTGCGTCCACGTTGTTATGGTTGTAGGAGT5700.12937227211755173No Hit
TGGCTGTTCGTTATCTACCTCGATGATTGCAACGCACGCACCAC5600.12710258313303327No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5580.12664864533612957No Hit
ACCGTGGTCGCCGTTGTCGCCATCGTCACCATCGTCGCCGTCAT5350.12142836067173715No Hit
CAGGAAGTTTTCTACAGAGGATTCAGTGCGAGAGTGGTAGTTTT5310.12052048507792978No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT5300.12029351617947792No Hit
ATCCTTCATCAGCAGTTCTTCGGAAAACAGGGCATCATACGGCA5230.118704733890315No Hit
TTTCACGTGTTCATACTTAGAAACATCGAAACCGTCCTGTTTGG5110.11598110710889288No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5030.11416535592127809No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5020.11393838702282626No Hit
TTCATCACCATCACCGCCTTCGTCACCGTCATCGCCGTCATCGC4940.11212263583521151No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG4900.11121476024140411No Hit
TTCTTCCAGTGCGCTCAGTTTAGAGTATGGAATGAAATGATAGT4890.11098779134295228No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG4820.10939900905378935No Hit
GATTTCGTTAACGATATCGCTGTCCTTCAGCTGATAGTTCGGGT4730.10735628896772276No Hit
CTCCTGACCTTCTTCACCTTCATCACCATCACCGCCTTCATCAC4580.10395175549094508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)