FastQCFastQC Report
Wed 8 May 2024
EGAF00007959929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007959929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440589
Total Bases13.6 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39020.8856326417590997No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT33330.7564873385399999No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC30700.6967945182471647No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG30100.6831763843400539No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG30010.6811336642539874No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG26010.5903461048732492No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC25130.5703728418094869No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC24910.5653795260435462No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG23440.532015097971125No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG23170.5258869377129252No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA22970.5213475597438882No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG19140.4344184716368316No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19010.4314678759569576No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG18660.42352396451114305No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC18490.4196654932374617No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17860.40536645263499543No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG16220.3681435532888928No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG15820.35906479735081903No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA15230.3456736323421602No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC14340.32547340037994593No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT14310.3247924936845904No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14130.3207070535124572No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13530.3070889196053465No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12800.29052019001836177No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12650.2871156565415841No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12460.28280324747099905No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA12440.2823493096740954No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC12430.2821223407756435No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG12350.28030658958802873No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG11780.26736936237627357No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG11610.2635108911025922No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA11470.2603333265242664No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA11170.25352425957071106No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT11070.2512545705861926No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG10600.24058703235895584No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG9820.22288345827971195No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9770.22174861378745273No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG9700.22015983149828977No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG9270.21040016886486046No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG9260.21017319996640862No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9220.20926532437260123No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG9160.20790351098189017No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9010.2044989775051125No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC8530.1936044703794239No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG8520.19337750148097207No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8500.19292356368406838No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG8340.18929206130883885No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC8270.18770327901967593No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC8190.18588752783206117No Hit
TCTGCGGACGGCGTAAACTGGACCACTCTGT8080.18339086994909087No Hit
AACATCTTTACCCCGATCAAGAAACCGGGCA7630.17317726951875784No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG7170.16273670018997297No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7060.16024004230700267No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC6950.15774338442403238No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6760.1534309753534473No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6750.15320400645499546No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6590.14957250407976597No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6560.1488915973844104No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6460.14662190839989195No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6350.14412525051692165No Hit
GGCCCGGCTCACTCCAAAGAAGTTCCGGCAC6040.13708921466491447No Hit
TCCCAGTCTTCCCAGAAATCCGAGAACCTGA6010.13640830796955894No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC5960.1352734634772997No Hit
CGCGATAACAACATTGAACGTGACAACAATA5960.1352734634772997No Hit
CTGCACGACGACGCTAACTTTCTGGAACATG5890.13368468118813678No Hit
GAAGCCGTGAACCGTGCGGTGGCTCGTGTCG5610.12732955203148513No Hit
TGGATCGAAGTATCCGGCCAGGAGCTGCCGA5550.12596773864077404No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA5550.12596773864077404No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5480.12437895635161113No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5410.12279017406244823No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5410.12279017406244823No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5190.11779685829650763No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC5190.11779685829650763No Hit
GGTCCAGCTCACTCTAAAGAAGTGCCGGCGC5030.11416535592127809No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5010.11371141812437442No Hit
TACTATAACGGTAAAGTTCCGTATGATGCAC4950.11234960473366334No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4900.11121476024140411No Hit
CAGATCGTGATCAAAATTACCGATCAGGGTT4870.11053385354604858No Hit
GTTGACTCCCTGCACGATGACGGCGATGACG4770.10826416456153012No Hit
AAAGAGCTGTACGATTCCCCGTCTGCGCAGC4770.10826416456153012No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG4670.10599447557701168No Hit
GCCCAGTCCGCGACCCCGTCTGTTTCTTCCT4470.10145509760797478No Hit
ACCCGTAAACATCGTGTGGTTGCTCTGAAAC4420.10032025311571555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)