FastQCFastQC Report
Wed 8 May 2024
EGAF00007960031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469902
Total Bases14.5 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT50491.0744793595260287No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26630.5667139105600743No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12730.27090755093615265No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG11370.24196534596575453No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10350.22025869223795602No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9840.2094053653740567No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9800.20855412405139795No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9720.20685164140608042No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9540.20302105545411597No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG9530.2028082451234513No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG9150.19472145255819298No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9040.1923805389208814No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7930.1687585922171006No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7820.16641767857978898No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7550.1606717996518423No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7510.15982055832918354No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA7080.1506697141106018No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6810.14492383518265511No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6800.14471102485199042No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6760.14385978352933165No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6500.13832671493204965No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG6430.13683704261739682No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5770.12279156079352715No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA5710.12151469880953901No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5680.12087626781754493No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA5650.12023783682555086No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5560.11832254384956864No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5530.11768411285757456No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC5470.11640725087358642No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5390.11470476822826886No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5280.11236385459095727No Hit
GTGCGTGCATCTAACCTGTCCGCCGAACAGC5180.11023575128431035No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5100.1085332686389928No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4960.10555392400968713No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA4890.10406425169503428No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4840.10300020004171083No Hit
CTGATCCTGGGCCTGGTTCTGGAACAGGTGC4770.10151052772705799No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)