FastQCFastQC Report
Wed 8 May 2024
EGAF00007960033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444413
Total Bases13.7 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT50421.1345302680164622No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39090.8795872307965789No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25810.580766089200811No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12280.27631954960813476No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG10350.23289147707200283No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10130.2279411268347236No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9270.20858975772535907No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9100.20476448708746145No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8780.19756397765141884No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8650.19463877069302654No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8560.19261362741413957No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG8100.18226289509982832No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7640.17191216278551708No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7260.16336155783021647No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7180.16156143047120583No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6750.1518857459165236No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6670.15008561855751296No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6660.14986060263763662No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6270.1410849817624597No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6260.14085996584258337No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG6080.13680967928480942No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6010.13523456784567509No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5970.13433450416616977No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5760.1296091698487668No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5550.12488383553136385No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5500.12375875593198218No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5360.12060853305371357No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5250.11813335793507392No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA5210.11723329425556857No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC5150.11588319873631059No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4850.10913272114002066No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA4600.10350732314311237No Hit
GTGCGTGCATCTAACCTGTCCGCCGAACAGC4540.10215722762385439No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC4510.10148217986422539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)