FastQCFastQC Report
Wed 8 May 2024
EGAF00007960042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429513
Total Bases18.8 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC35140.8181358887856711No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT31760.7394421123458428No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA30170.7024234423637934No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA29120.6779771508662136No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA27100.6309471424613458No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT25890.6027757017831824No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA25870.6023100581356094No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT24650.5739057956336595No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT21750.5063874667355819No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA20310.4728611241103296No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA20280.4721626586389702No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG20090.46773904398702715No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT19580.45586513097391695No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG15710.3657630851685514No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT15290.35598456856951943No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT14780.34411065555640924No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14640.3408511500233986No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT13250.3084889165170786No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG13190.3070919855743598No Hit
AACGTTCAGCGCTACGAACTGTGCTTTCGCCATAGCGTCAGAAA11750.27356564294910746No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG11320.2635543045262891No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT11250.26192455175978374No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC11050.25726811528405424No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG10710.24935217327531414No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9910.23072642737239618No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC9640.22444023813016134No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG9590.22327612901122898No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG9330.21722276159278067No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT9310.2167571179452077No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT9290.21629147429763476No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC8980.20907399776025404No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA8950.2083755322888946No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8440.19650161927578444No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT7570.17624612060636116No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7420.17275379324956405No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT7180.16716606947868867No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6960.16204398935538622No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA6690.1557578001131514No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6610.1538952255228596No Hit
CAGTGCCTGCAGGTCTTCAGTTTTCATGTAGCCGGACAGGCCAG6550.15249829458014078No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6220.1448151743951871No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC6170.14365106527625474No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC6080.14155566886217646No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6040.14062438156703058No Hit
AGAACGACGGGAACGGCGGTTCGGTTTGGAACGCTTAGAGTTAT5790.1348038359723687No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC5600.1303802213204257No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5520.12851764673013388No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA5510.1282848249063474No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG5500.12805200308256096No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5490.12781918125877445No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5400.1257237848446962No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA5270.12269710113547204No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5270.12269710113547204No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT5130.1194375956024614No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG5000.11641091189323721No Hit
CGGTTCACCATCCGGACCACCTTCCTGGTGATATTCAAAGTAGT4940.11501398095051837No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4700.109426257179643No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT4650.10826214806071062No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT4420.10290724611362172No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC4410.10267442428983523No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT4400.10244160246604876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)