FastQCFastQC Report
Wed 8 May 2024
EGAF00007960043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429513
Total Bases13.3 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT65211.5182311129116No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC53961.256306561151816No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG34960.8139450959575146No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA30910.7196522573239925No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT29940.6970685404167045No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG26600.6193060512720221No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT25080.5839171340564779No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC23300.5424748494224855No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22730.5292040054666565No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG22380.5210552416341299No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC21420.4987043465506283No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA20110.46820468763460016No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG18700.4353768104807072No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC16920.39393452584671473No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC16010.3727477398821456No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT15000.34923273567971164No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14700.3422480809661174No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC13710.31919872041125646No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA12150.28287851590056645No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11930.277756435777264No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA11840.27566103936318576No Hit
GAAACCACTATCGCAGATAATAGCAAACGCT11830.2754282175393993No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA11410.26564970094036733No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11190.26052762081706493No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11100.25843222440298663No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA10470.24376444950443876No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG10270.23910801302870927No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC10170.23677979479084454No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10000.23282182378647442No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT9880.23002796190103675No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT9570.22281048536365605No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG8870.2065129576986028No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8840.20581449222724343No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8250.19207800462384142No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA8130.18928414273840374No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8090.18835285544325783No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA7910.1841620626151013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7820.18206666620102302No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7600.17694458607772057No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7170.16693324765490217No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7070.16460502941703742No Hit
GAAAACGCTGCGTACTATCAGGTAGTTCCGG6440.14993725451848955No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG6300.1466777489854789No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6060.14109002521460354No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6030.1403915597432441No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5810.13526947961994165No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5690.13247561773450395No Hit
AAAGCGAACACCGTTGCAGACAACTCTGTAG5670.132009974086931No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5670.132009974086931No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5520.12851764673013388No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5410.12595660666848268No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5380.12525814119712325No Hit
GGTTCTCGTGAGCGTGCACGCGGCCGTGGCC5370.1250253193733368No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA5160.12013606107382081No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5090.1185063083073155No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5000.11641091189323721No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4830.11245294088886716No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4740.1103575444747889No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA4640.10802932623692416No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG4580.1066323952942053No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC4340.10104467152332991No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)