FastQCFastQC Report
Wed 8 May 2024
EGAF00007960044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406398
Total Bases17.8 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC33700.8292363643521868No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT30770.7571395528521302No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA28270.6956235020841638No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA28160.6929167958503732No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA25800.634845643925413No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA24460.601873040713783No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT24280.5974438850584894No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT22340.5497074296625475No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT21110.519441532684708No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA19380.4768724255532754No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA18840.4635849585873947No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG18570.4569412251044543No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT18440.45374239046452003No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT15130.37229513924773255No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG14780.36368289214021726No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13920.3425213706760368No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT13860.3410449854576056No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG12620.31053302427669427No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT12450.30634993282447254No Hit
AACGTTCAGCGCTACGAACTGTGCTTTCGCCATAGCGTCAGAAA11270.2773143568619924No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT11000.27067062337905207No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC10550.2595977342408181No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG10510.25861347742853064No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG9600.2362216349489909No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9330.22957790146605053No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC9060.22293416798311017No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG8960.2204735259523915No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT8950.22022746174931965No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG8650.2128455356571637No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA8650.2128455356571637No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC8570.21087702203258876No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8450.20792425159572636No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT8390.20644786637729517No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT7350.18085718925782115No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6900.1697843001195872No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6830.16806185069808416No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6780.16683152968272483No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT6540.16092598880900005No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6260.1540361911229878No Hit
CAGTGCCTGCAGGTCTTCAGTTTTCATGTAGCCGGACAGGCCAG6250.15379012691991595No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA6160.15157554909226914No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC5880.14468575140625692No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC5840.14370149459396944No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5590.1375498895171728No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5540.13631956850181348No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5490.13508924748645415No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA5480.1348431832833823No Hit
AGAACGACGGGAACGGCGGTTCGGTTTGGAACGCTTAGAGTTAT5360.1318904128465199No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5320.13090615603423245No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA5270.12967583501887311No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG5250.12918370661272938No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5130.126230936175867No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT4890.12032539530214223No Hit
CGGTTCACCATCCGGACCACCTTCCTGGTGATATTCAAAGTAGT4870.11983326689599849No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4840.11909507428678291No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT4830.11884901008371106No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA4770.11737262486527986No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG4690.11540411124070493No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4300.10580760732090218No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT4300.10580760732090218No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG4120.10137845166560859No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC4090.100640259056393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)