FastQCFastQC Report
Wed 31 May 2023
EGAF00007960045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406398
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT60391.4859817223509957No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC49861.2268761165163213No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39260.9660480612601439No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG31950.7861751288146103No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA29400.7234287570312846No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT28520.7017751071609605No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG24860.6117156088366577No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT22760.5600421261915659No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC22280.5482310444441163No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20900.5142741844201989No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG20600.506892258328043No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC20020.4926205345498747No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA18670.45940186713517295No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG16890.4156024389883809No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC15640.3848444136043977No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT14870.36589746996786404No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC14690.3614683143125704No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13510.3324327383500903No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC12600.31004089587055056No Hit
GAAACCACTATCGCAGATAATAGCAAACGCT11570.2846962829541484No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA11490.28272776932957344No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10990.2704245591759802No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA10860.2672257245360459No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA10810.2659954035206866No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT10790.26550327511454286No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10360.25492251438245267No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9740.23966653379199698No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT9300.22883970885683494No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA9220.22687119523226001No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG9110.2241644889984695No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8990.22121171856160712No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8690.21382979246945114No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8490.20890850840801384No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG8480.20866244420494195No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT8270.2034950959404328No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA7670.18873124375612085No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7450.18331783128853982No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7330.18036506085167742No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA7250.1783965472271025No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG6620.16289450243357498No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6350.1562507689506346No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6340.15600470474756273No Hit
GAAAACGCTGCGTACTATCAGGTAGTTCCGG6120.1505912922799817No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5610.13804201792331655No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5590.1375498895171728No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA5540.13631956850181348No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5530.13607350429874163No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5430.13361286226802296No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5380.13238254125266363No Hit
GGTTCTCGTGAGCGTGCACGCGGCCGTGGCC5370.13213647704959178No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5330.1311522202373043No Hit
AAAGCGAACACCGTTGCAGACAACTCTGTAG5250.12918370661272938No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5050.12426242255129208No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5040.12401635834822021No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4830.11884901008371106No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4780.11761868906835173No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4630.11392772602227375No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4320.1062997357270459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)