FastQCFastQC Report
Wed 31 May 2023
EGAF00007960046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433568
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC35540.8197099416931138No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT33120.7638940143183999No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA29770.6866281644401802No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA29210.6737120820724777No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA27740.6398073658572588No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA26020.6001365414421729No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT25720.5932172116023322No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT24660.5687689128348956No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT21400.493578861908628No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA20750.4785869805889733No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA20720.4778950476049893No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG19780.4562144807734888No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT18840.43453391394198837No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG15720.36257288360764633No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT15240.35150195586390137No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14940.3445826260240608No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT14520.33489556424828404No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG14240.3284375230644328No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT12870.29683925012916085No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT12360.2850763894014318No Hit
AACGTTCAGCGCTACGAACTGTGCTTTCGCCATAGCGTCAGAAA11660.2689312864418038No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG11580.26708613181784635No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC11190.2580910030260536No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG10760.2481732969222821No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG10030.23133626097867002No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG9960.2297217506827072No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT9650.22257177651487195No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC9430.21749760129898885No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC9400.2168056683150048No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC9240.21311535906708984No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT9170.21150084877112701No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT9010.20781053952321202No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT7920.18267030777179127No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7750.17874935419588162No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7600.17528968927596134No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT7230.16675584914015795No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA6970.1607590966122961No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6770.1561462100524024No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6720.15499298841242898No Hit
CAGTGCCTGCAGGTCTTCAGTTTTCATGTAGCCGGACAGGCCAG6720.15499298841242898No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC6680.15407041110045022No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6450.14876559155657243No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6310.14553657096464684No Hit
AGAACGACGGGAACGGCGGTTCGGTTTGGAACGCTTAGAGTTAT6040.13930917410879032No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6000.1383865967968116No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA5940.13700273082884346No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5900.1360801535168647No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA5840.1346962875488966No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5830.1344656432209019No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC5780.1333124215809285No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5370.12385600413314635No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG5240.12085762786921544No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG5230.12062698354122076No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5200.1199350505572367No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5090.11739796294929516No Hit
CGGTTCACCATCCGGACCACCTTCCTGGTGATATTCAAAGTAGT4970.11463023101335892No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC4730.10909476714148646No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4730.10909476714148646No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT4640.10701896818953427No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG4620.10655767953354492No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA4560.10517381356557678No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT4460.10286737028562994No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4400.10148350431766183No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT4400.10148350431766183No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT4390.10125285998966714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)