FastQCFastQC Report
Wed 8 May 2024
EGAF00007960047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433568
Total Bases13.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT63701.4692043693261496No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC55351.2766163554505867No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG34260.7901874677097942No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT31250.7207635249833936No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA30990.7147667724555318No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG26740.6167429330577903No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT25460.5872204590744705No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC23360.5387851501955864No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT23180.5346335522916821No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG22770.5251771348438999No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC21030.48504502177282455No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA19590.45183223854158977No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC17820.4110081924865304No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17690.40800981622259946No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC17050.39324857923093953No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT15050.34711971363200234No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14850.34250682707210867No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC13530.3120617757768101No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA12600.2906118532733043No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA12270.28300059044947967No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12100.27907963687356996No Hit
GAAACCACTATCGCAGATAATAGCAAACGCT11920.27492803896966567No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11730.27054579673776663No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11040.25463133810613325No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11010.25393940512214924No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA10750.2479426525942874No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10670.24609749797032993No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG10270.23687172485054248No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT9730.22441693113882943No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC9510.21934275592294636No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9450.21795888995497825No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG9250.2133460033950845No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT9210.21242342608310577No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8850.20412023027529708No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA8480.1955863901394937No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8310.19166543656358404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8090.18659126134770093No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA7920.18267030777179127No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7510.17321389032400913No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7340.1692929367480995No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG7050.1626042512362536No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7030.16214296258026423No Hit
GAAAACGCTGCGTACTATCAGGTAGTTCCGG6600.15222525647649274No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6210.14323012768469998No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6160.14207690604472656No Hit
AAAGCGAACACCGTTGCAGACAACTCTGTAG6110.1409236844047531No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5800.13377371023691786No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA5790.13354306590892318No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5690.1312366226289763No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5640.13008340098900287No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5540.12777695770905603No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5430.12523987010111448No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5300.12224149383718355No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA5280.12178020518119419No Hit
GGTTCTCGTGAGCGTGCACGCGGCCGTGGCC5270.1215495608531995No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5250.12108827219721012No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5120.11808989593327922No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC4900.11301572071739613No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG4670.10771090117351835No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA4500.10378994759760868No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG4460.10286737028562994No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4460.10286737028562994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)