FastQCFastQC Report
Sun 21 May 2023
EGAF00007960048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409498
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC33180.8102603675720028No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT30720.7501868140992142No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA28850.7045211454024196No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA28410.6937762821796444No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA24560.5997587289803613No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA24320.5938978944952112No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT23050.5628843120112919No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT22890.5589770890211918No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT21110.5155092332563285No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA19050.46520373725879No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA19040.46495953582190874No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT19020.4644711329481463No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG18300.446888629492696No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG15970.3899896946993636No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT15090.3684999682538132No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14660.3579993064679193No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT14360.35067326336148164No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG12470.3045191917909245No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT12220.29841415586889314No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC11210.27374981074388643No Hit
AACGTTCAGCGCTACGAACTGTGCTTTCGCCATAGCGTCAGAAA11060.27008678919066764No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT10680.2608071345891799No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG10470.25567890441467356No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG10400.2539694943565048No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG9850.2405384153280358No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8870.21660667451367283No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG8650.21123424290228526No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC8510.20781542278594767No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT8500.20757122134906644No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT8420.20561760985401636No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC8390.20488500554337263No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8250.20146618542703507No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7390.18046486185524716No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7380.1802206604183659No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT6990.17069680437999696No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA6930.16923159575870944No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT6750.16483596989484686No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6660.1626381569629156No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6640.16214975408915305No Hit
CAGTGCCTGCAGGTCTTCAGTTTTCATGTAGCCGGACAGGCCAG6580.16068454546786554No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6320.1543353081089529No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC6190.1511606894294966No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT5860.1431020420124152No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA5670.13846221471167136No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5530.13504339459533382No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5520.13479919315845254No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC5480.13382238741092753No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5390.13162457447899623No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5300.12942676154706495No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA5280.12893835867330244No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5200.1269847471782524No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG5190.12674054574137114No Hit
AGAACGACGGGAACGGCGGTTCGGTTTGGAACGCTTAGAGTTAT5080.12405432993567735No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG4900.11965870407181477No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT4670.11404207102354591No Hit
CGGTTCACCATCCGGACCACCTTCCTGGTGATATTCAAAGTAGT4630.11306526527602087No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4580.1118442580916146No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG4270.10427401354829573No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT4230.10329720780077069No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC4210.10280880492700818No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT4210.10280880492700818No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4110.10036679055819564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)