FastQCFastQC Report
Wed 31 May 2023
EGAF00007960049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409498
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT58761.4349276431142521No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC51601.2600794143072738No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37180.9079409423245046No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG32420.7917010583690275No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA29090.7103819798875697No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT28150.6874270448207318No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG23740.5797342111560984No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT23450.5726523694865421No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC22720.5548256645942105No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20920.5108694059555847No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG20210.4935311039370156No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC19310.4715529746177027No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA19180.46837835593824634No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG18220.44493501799764584No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC16600.40537438522288266No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC15340.3746050041758446No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14420.35213847198276915No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT13960.34090520588623147No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC12680.3096474219654308No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA11540.28180845816096783No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT11370.2776570337339865No Hit
GAAACCACTATCGCAGATAATAGCAAACGCT11270.27521501936517395No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA11140.2720404006857176No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA10560.2578767173466049No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10380.2534810914827423No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9980.24371303400749209No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG9810.2395616095805108No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9560.23345657365847944No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC9350.22832834348397307No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA9100.2222233075619417No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT8880.21685087595055408No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT8810.21514146589238528No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG8460.20659441560154138No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8450.20635021416466015No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7700.18803510639856605No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7670.1873025020879223No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA7270.1775344446126721No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7240.17680184030202833No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA7200.17582503455450332No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7010.1711852072537595No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG6780.16556857420549062No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6700.1636149627104406No Hit
GAAAACGCTGCGTACTATCAGGTAGTTCCGG6440.15726572535152797No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5770.14090422908048392No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5740.14017162476984013No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5560.13577599890597755No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5510.1345549917215713No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5490.13406658884780878No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA5400.1318687759158775No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA5270.1286941572364212No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5260.1284499557995399No Hit
AAAGCGAACACCGTTGCAGACAACTCTGTAG4940.12063550981933978No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4930.12039130838245854No Hit
GGTTCTCGTGAGCGTGCACGCGGCCGTGGCC4810.11746089113988346No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4570.11160005665473335No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC4570.11160005665473335No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC4530.11062325090720833No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4390.10720443079087077No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG4100.1001225891213144No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)