FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007960051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483648
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT100642.0808521900224957No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA65861.3617341537647214No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC45590.9426276961757312No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA35100.725734418420008No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA32890.6800400291120815No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA27600.5706629614926558No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG26040.5384080984517665No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21420.4428840809845177No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT21230.43895560407569145No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17110.3537696837369326No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17010.3517020643112346No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15190.31407139076353047No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13250.2739595739049887No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC13120.2712716686515813No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12190.25204280799258966No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC11770.24335880640465793No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10840.22412994574566628No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10560.21834061135371177No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10410.21523918221516475No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT10370.21441213444488555No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9700.20055908429270874No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9350.19332241630276567No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9090.1879466057959508No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8470.175127365356623No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7680.15879317189360856No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7470.15445117109964274No Hit
TTCCCTCAGCCGCAGCAGCCATTCCCGCAAA7120.1472145031096996No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA7070.1461806933968506No Hit
AACGAATACAAATGGCCGTCCCAGCACCGTT6980.14431983591372238No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6670.1379102156940585No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6460.13356821490009263No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6370.1317073574169644No Hit
CTGCAGCCGCAACAGCCGCTGCCGCAGCAGC5760.11909487892020643No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5690.1176475453222178No Hit
TTTCTGCAGCAGCCGGGTCAAATCATTCCAC5610.1159934497816594No Hit
GACCCATTCCTGGACGGCTCTCACTCTGTTA5600.11578668783908959No Hit
CAGCCGTTCCCGCTGCAGCCACAGCAGCCGT5580.11537316395394998No Hit
CTGCCGTTCCCGCAGCAGCCGCAGCAGCCGT5510.11392583035596136No Hit
CGTCAGAAACCGAACAACAACAACAACAAGA5410.11185821093026332No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5330.11020411538970491No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5130.10606887653830885No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4850.10027954214635437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)