FastQCFastQC Report
Wed 8 May 2024
EGAF00007960052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458773
Total Bases20.1 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA40590.8847512822245425No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA37460.8165258199588903No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC29250.637570214463362No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT29250.637570214463362No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC28470.6205683420776724No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA24230.5281479075708466No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC23590.5141976533056652No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA21380.4660256815462113No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT19110.41654587344939653No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA16870.3677199835212622No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG15520.33829366593064547No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT15050.32804894795465295No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA14730.3210738208220623No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14620.3186761208702343No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC14390.3136627482436848No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC11970.26091334930346816No Hit
AACAGCGGTAGACGGAGCGGAAGAGGCCAGAGCGCCAGTACCCG11540.25154052221904954No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG10860.2367183770622944No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10130.22080636829107209No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9770.2129593502669076No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC9250.20162476867644782No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9120.19879112327883286No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8560.18658465079679928No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT8450.18418695084497125No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA8100.17655790554370027No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7970.1737242601460853No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7840.17089061474847037No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6980.15214496057963306No Hit
ACCGCGAGCCGCGATACGACGCATTGCTTCCACCTGCTGGAAAC6730.14669564250729664No Hit
CGGAGAACACTGCTGCAGCAGTACGTTTTTGCATGGGTTCAGCT6350.13841267903734528No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG5960.12991174284450044No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT5760.1255522883866313No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5750.12533431566373784No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5700.12424445204927055No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5660.12337256115769672No Hit
CTGCGGCTGTTGCGGAGATGGCTGCTGAGGCTGCAGCAGGAACG5550.1209748612058687No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA5390.11748729763957338No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5340.11639743402510609No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT5310.11574351585642573No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC5220.11378176135038462No Hit
CTGCAGGTACGGCTGCTGCGGCTGGTGCGGCTGCTGCGGGGATG5180.11290987045881079No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5130.1118200068443435No Hit
CTGCAGGTACGGTTGGATCAGCGGTTGCTGTTGCTGCGGGAAGC5030.10964027961540893No Hit
CACTACAACAACGAACGGTTGCTGAGGCTGCGGGAACGGCAGCT5010.10920433416962201No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG4930.10746055238647437No Hit
CGGGCACTGCGGAACGGTCAGGTACAGGCCGCGACGTTTTTCCG4640.10113934342256412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)