FastQCFastQC Report
Wed 31 May 2023
EGAF00007960053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458773
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT94362.056790613222661No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA62231.3564442545659836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43930.9575541716709571No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC42020.9159213815983068No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA32990.719092012825515No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA30760.670484095620274No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA24920.543188025450495No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG24390.5316354711371419No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19600.42722653687117595No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT18780.4093527735939124No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15790.34417892944876877No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15460.3369858295932847No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14280.31126504829185675No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12560.27377373995418214No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC12430.2709400945565672No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11580.25241241311062335No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC11430.24914282226722148No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10390.22647365908630193No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9990.21775475017056364No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9780.21317732298980102No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9500.20707408674878425No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9420.20533030496563662No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8940.19486761426675067No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7970.1737242601460853No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7390.1610818422182648No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6760.14734956067597701No Hit
AACGAATACAAATGGCCGTCCCAGCACCGTT6590.14364402438678825No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6580.1434260516638948No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA6370.13884862448313218No Hit
TTCCCTCAGCCGCAGCAGCCATTCCCGCAAA6360.13863065176023873No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6260.13645092453130414No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6170.13448917002526303No Hit
CAGCCGTTCCCGCTGCAGCCACAGCAGCCGT5410.11792324308536029No Hit
CTGCAGCCGCAACAGCCGCTGCCGCAGCAGC5320.11596148857931918No Hit
TTTCTGCAGCAGCCGGGTCAAATCATTCCAC5320.11596148857931918No Hit
CTGCCGTTCCCGCAGCAGCCGCAGCAGCCGT5010.10920433416962201No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4990.1087683887238351No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4980.10855041600094165No Hit
GACCCATTCCTGGACGGCTCTCACTCTGTTA4970.1083324432780482No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4880.10637068877200706No Hit
CGTCAGAAACCGAACAACAACAACAACAAGA4820.10506285243464632No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC4780.1041909615430725No Hit
CCGCAGCAGCTGTTTCCGCTGCCGCAGCAGC4720.10288312520571176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)