FastQCFastQC Report
Wed 8 May 2024
EGAF00007960054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489322
Total Bases21.5 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA44280.9049255909196807No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA41790.8540388537609183No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC31330.6402736848128635No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT31070.6349602102501012No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC30170.616567413686693No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA25640.5239903376508721No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC25150.5139764817441276No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA22870.46738139711682697No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT20880.42671288027106896No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA19100.3903360159567728No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG16480.33679254151662913No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT16410.3353619906728085No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA15850.3239175839222434No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT15590.3186041093594811No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC15220.31104262632785773No Hit
AACAGCGGTAGACGGAGCGGAAGAGGCCAGAGCGCCAGTACCCG12820.26199516882543605No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC12400.25341186376251223No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG11240.22970559263634172No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT11150.2278663129800009No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10100.20640805032269138No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9950.20334258422879006No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC9610.19639419441594697No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT9110.18617597410294243No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8730.178410126665059No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8220.16798754194579438No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT8000.1634915250080724No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7530.15388639791384814No Hit
CGGAGAACACTGCTGCAGCAGTACGTTTTTGCATGGGTTCAGCT7530.15388639791384814No Hit
ACCGCGAGCCGCGATACGACGCATTGCTTCCACCTGCTGGAAAC7080.14468999963214407No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT6540.1336543216940992No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG6280.12834084713133684No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6260.12793211831881665No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5930.12118809291223366No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5790.1183269912245924No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT5770.1179182624120722No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5770.1179182624120722No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA5660.11567025394321122No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5650.11546588953695112No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5640.11526152513069104No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC5620.11485279631817086No Hit
CTGCGGCTGTTGCGGAGATGGCTGCTGAGGCTGCAGCAGGAACG5550.11342224547435023No Hit
CGGGCACTGCGGAACGGTCAGGTACAGGCCGCGACGTTTTTCCG5530.11301351666183006No Hit
CTGCAGGTACGGTTGGATCAGCGGTTGCTGTTGCTGCGGGAAGC5460.1115829658180094No Hit
CTGCAGGTACGGCTGCTGCGGCTGGTGCGGCTGCTGCGGGGATG5330.10892622853662824No Hit
CACTACAACAACGAACGGTTGCTGAGGCTGCGGGAACGGCAGCT5040.1029996607550856No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG4980.10177347431752506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)