FastQCFastQC Report
Wed 31 May 2023
EGAF00007960055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489322
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT98602.0150330457244925No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA67611.381707750724472No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC45370.9272013112020305No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA35100.7173190659729176No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA33550.6856425830026036No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA27270.5573017358712667No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG26180.5350260155889168No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG22090.45144097342853984No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT21510.4395878378654547No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC17660.3609075414553198No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17450.3566158889238579No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15510.3169691941094004No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC13890.2838621602952657No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13240.2705784738883598No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC12490.255251143418853No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG12330.2519813129186916No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11090.22664012654244037No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10950.22377902485479906No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10850.2217353807921982No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9950.20334258422879006No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9580.19578110119716668No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9380.1916938130719649No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9210.18821961816554333No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8500.1737097453210769No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8320.17003118600839529No Hit
TTCCCTCAGCCGCAGCAGCCATTCCCGCAAA7540.15409076232010824No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7480.1528645758825477No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA7330.14979910978864633No Hit
AACGAATACAAATGGCCGTCCCAGCACCGTT7330.14979910978864633No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6720.13733288100678082No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6500.1328368640690588No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6440.13161067763149828No Hit
CTGCAGCCGCAACAGCCGCTGCCGCAGCAGC5820.11894008444337267No Hit
GACCCATTCCTGGACGGCTCTCACTCTGTTA5750.11750953359955203No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5690.11628334716199148No Hit
CTGCCGTTCCCGCAGCAGCCGCAGCAGCCGT5590.11423970309939059No Hit
CAGCCGTTCCCGCTGCAGCCACAGCAGCCGT5490.1121960590367897No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5450.11137860141174932No Hit
TTTCTGCAGCAGCCGGGTCAAATCATTCCAC5450.11137860141174932No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5370.1097436861616686No Hit
CGTCAGAAACCGAACAACAACAACAACAAGA5360.10953932175540851No Hit
CCGCAGCAGCTGTTTCCGCTGCCGCAGCAGC5000.10218220313004524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)