FastQCFastQC Report
Wed 8 May 2024
EGAF00007960057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464733
Total Bases14.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT94312.029337275381778No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA62041.3349600738488552No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC43240.9304267181370809No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40660.874910970385146No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA33440.7195529476064751No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA31740.6829728037389211No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA25550.5497780445976508No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG24480.5267540716927784No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19930.42884839251785434No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19360.4165832854563803No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG15720.338258742116441No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15440.33223377724413805No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13900.2990964704464714No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC12930.27822427071027883No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12750.27435107900665545No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11730.252402992686123No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC11660.25089675146804724No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10030.21582284881856892No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9850.21194965711494557No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9800.21087377053060574No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9800.21087377053060574No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9550.20549433760890662No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8600.18505249250644995No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7820.16826866179074867No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7440.160091923749766No Hit
TTCCCTCAGCCGCAGCAGCCATTCCCGCAAA6840.1471812847376881No Hit
AACGAATACAAATGGCCGTCCCAGCACCGTT6740.14502951156900842No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6710.14438397961840455No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA6520.1402956105979132No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6360.13685277352802577No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5890.1267394396352314No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5790.12458766646655177No Hit
CTGCAGCCGCAACAGCCGCTGCCGCAGCAGC5620.12092965207979635No Hit
TTTCTGCAGCAGCCGGGTCAAATCATTCCAC5420.11662610574243706No Hit
GACCCATTCCTGGACGGCTCTCACTCTGTTA5330.11468950989062536No Hit
CGTCAGAAACCGAACAACAACAACAACAAGA5270.11339844598941759No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5090.10952525428579421No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4980.10715830380024659No Hit
CTGCCGTTCCCGCAGCAGCCGCAGCAGCCGT4890.10522170794843491No Hit
CAGCCGTTCCCGCTGCAGCCACAGCAGCCGT4860.10457617599783102No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4820.10371546673035915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)