FastQCFastQC Report
Wed 8 May 2024
EGAF00007960066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421471
Total Bases18.5 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA39280.9319739673666753No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA36780.8726579052888573No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT25550.6062101544352992No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC20080.4764266106090336No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT19560.46408886969684743No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG18060.4284992324501567No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG17260.40951809258525496No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA15360.3644378854061134No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG15340.36396335690949083No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT15290.36277703566793446No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14290.3390506108368073No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA14260.33833881809187344No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG14100.3345425901188931No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG13410.3181713569854154No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG12950.3072572015630969No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12710.30156285960362633No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT12550.297766631630646No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12000.284717097973526No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG11200.2657359581086243No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG10810.2564826524244847No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT10590.25126283896163676No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC10230.24272132602243096No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC9860.2339425488349139No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT9610.2280109426271321No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT9350.22184207217103907No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC9340.2216048079227278No Hit
GCGTTCCAGGACGTTACCTTGGGAACGGTTCTCCGCATCACGCT9340.2216048079227278No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG9310.22089301517779397No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8990.21330055923183328No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG8840.2097415955071642No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG8790.20855527426560783No Hit
TTCCATCGGCTGCTGCGGGCTTACCTGGGTCAGCTGCGGTGGCT8540.20262366805782603No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC8330.19764111884328933No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT8060.19123498413888498No Hit
CGGGAAAGACAGATCTTCGTCAGAAGAAGAGCTGGATGCCGCAG8040.19076045564226246No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT8000.18981139864901736No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG7940.18838781315914974No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7890.1872014919175934No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG7840.18601517067603704No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7790.18482884943448066No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG7660.18174441420643414No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG7400.17557554375034107No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7370.17486375100540724No Hit
GTCACCGTCCCACAGGTAGGTGAAGCGGATCTTTTTGCGGTCGC6860.1627632743415324No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6810.16157695309997605No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT6760.16039063185841967No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6750.1601533676101084No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT6740.15991610336179712No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT6580.15611987538881678No Hit
GGACTGGTCCGGCACGTGCAGAACCGGCAGGTGCATGCGAGGCG6290.1492392121877899No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6240.14805289094623356No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA5870.1392741137587165No Hit
GCGCTGACGACGTTCCAGAACGTTGCCCTGGGATTTGTTTTCGA5760.1366642070272925No Hit
CGGCGGCAGCGGCATCGGAGGGATGTTGTAGAAAACGGTCGGGG5580.1323934505576896No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5530.13120712931613326No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5430.12883448683302054No Hit
GGACTGGCCACGGTGGCCACCATCGTAGCGTTCCCAGCCGCTCG5290.12551278735666274No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5270.1250382588600402No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG5150.12219108788030493No Hit
CGGGTCCTGGGACCACGGAGCCCACGGATGACGGTAGCACGGGT5120.12147929513537112No Hit
GCCACCATGGGCGCCTTTGCAACCGATACAAGACGGACGTTTCT5110.12124203088705984No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5070.12029297389381476No Hit
CATGGACTCTTCGTGCAGCAGTACTGCAGTAACTTGAGGGCCCG5050.1198184453971922No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG4740.1124632536995428No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT4730.11222598945123151No Hit
CAGCAGAAATTCCTCTTCACCGGATTCCATCGGCGCGTGCTTGC4730.11222598945123151No Hit
CTGCATCTGGTGCATAACGCGATGAGACGGCGCACGTGCCAGCA4710.11175146095460899No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4710.11175146095460899No Hit
GCCGCTAGCGTCGTGACGATATTCCCACTGCTCGCCCTGTGCAG4670.1108024039613639No Hit
CGGAGCCTGCGGTGGCGGCGGCTCTTGATAACCCTGATACGGCG4650.11032787546474135No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA4520.10724344023669481No Hit
TGCATCTTCGAAGTCTTCATCATCGTCGTCGTCTTCTTCTTCAC4500.10676891174007228No Hit
CGGCGAAGCGACCGTTGAAAAAGGTGGATGCAACTGATTTAAAT4480.10629438324344972No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA4470.10605711899513846No Hit
CAGGTTGTGAGATGCACGGGTACGACGGTCTTCCGGGGTATCGT4370.10368447651202574No Hit
CGTACTCCTGGCGACAGAGAAAATGGCGGTGACGTTATTTAAAT4300.10202362677384684No Hit
AACAGTCTGAATTGCCGCATCCACAGTGGAAACCGGCGGTGGGG4240.1006000412839792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)