FastQCFastQC Report
Wed 8 May 2024
EGAF00007960067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421471
Total Bases13 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT90162.1391744627744256No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC27900.6619672527884481No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA26340.6249540300518897No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20620.48923888001784227No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC19080.4527001857779064No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC18700.44368414434207815No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC15860.376301097821677No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15090.358031750701709No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14760.35020203050743703No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT14700.34877844501756944No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC14520.34450768854796654No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT14450.3428468388097876No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14230.3376270253469396No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG13870.32908551240773387No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13620.3231539061999521No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG13410.3181713569854154No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT12990.3082062585563419No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC12770.302986445093494No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12700.30132559535531506No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA12420.29468219640259946No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11500.27285388555796247No Hit
CGTCAGCGTGACGCAGAAAACCGTAGCCAGG10490.24889019647852403No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10240.24295859027074224No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC10170.24129774053256334No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9950.23607792706971534No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9890.23465434157984771No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9580.22729914988219832No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9570.22706188563388705No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9410.2232656576609067No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA9360.2220793364193503No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC9360.2220793364193503No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC9320.22113027942610525No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA9230.2189949011913038No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9000.21353782348014455No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC8780.20831801001729655No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8650.20523357478925003No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8430.20001376132640206No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA8370.1985901758365344No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8350.19811564733991188No Hit
GGTATGGATGAACCACCGTCTGGTTGGGAAC8310.19716659034666678No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA8070.19147224838719626No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7980.1893368701523948No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7920.1879132846625272No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7880.1869642276692821No Hit
TCTCGTACCAAACACCGTGACGGTTACCGTC7750.1838797924412356No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC7410.17581280799865234No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7080.16798308780438037No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6730.15967883911348588No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT6610.1568316681337506No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA6560.15564534689219425No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC6350.15066279767765753No Hit
GACGTAGAGAACAAATCTCAGGGTAACGTGC6290.1492392121877899No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6000.142358548986763No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5990.14212128473845176No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5860.13903684951040524No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5840.13856232101378269No Hit
GGTCACCGTCTGAAACAAGCAGGTAACACTC5810.13785052826884886No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5790.13737599977222634No Hit
CAGCCTACTCCGTCCCCGCCGCGCATGCACC5660.1342915645441798No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5620.1333425075509347No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5610.13310524330262344No Hit
GCTACCCCAGAAAGCCAGGCCCCAACCGTCG5560.13191892206106706No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5560.13191892206106706No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5510.1307326008195107No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5430.12883448683302054No Hit
GGTTGGGGTCGTCGCTACGAACCAGCACCGA5370.12741090134315292No Hit
CTGCGTGTATTCGCTTGTTGCTGCGTTAAAC5290.12551278735666274No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5180.12290288062523874No Hit
GGCGGTGATAACCACGGTCGCGGTCGTGGTC5120.12147929513537112No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT4800.11388683918941044No Hit
CGTGTTCGTGGTCACTACCGTGCTCCGTGGG4720.11198872520292023No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA4680.11103966820967515No Hit
GGTCGTCTGGGTGTCGGCCCGCAGGTAACCG4670.1108024039613639No Hit
CGCGCATCTATCGGTTCTAAAAGCGTCTCTA4470.10605711899513846No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4390.10415900500864828No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC4330.10273541951878065No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4300.10202362677384684No Hit
CGTGTTCGTGGCCACTATCGTGCGCCATGGG4300.10202362677384684No Hit
GCACCGAAGCCTTCCAAACAGAGCAAGAAGA4270.10131183402891301No Hit
ACCACCCTGCCGCCGACTCCGCTGCTGACCG4260.10107456978060175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)