FastQCFastQC Report
Wed 8 May 2024
EGAF00007960068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398857
Total Bases17.5 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA38020.9532238371145548No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA36850.9238900157199196No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT23810.5969558012019346No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC18430.46207036607104807No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT18180.4558024555166388No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG17770.44552308220740766No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG15220.3815903945524336No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA14410.3612823643561477No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG14310.358775200134384No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA13940.34949869251385834No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13760.34498579691468373No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT13560.33997146847115634No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG13410.3362107221385108No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12670.3176577068974595No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG12670.3176577068974595No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG12270.30762905001040475No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11280.2828081242149442No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT11000.2757880643940059No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG10230.2564828998864255No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG9850.24695567584372344No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC9790.24545137731066521No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG9440.23667630253449232No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC9420.2361748696901396No Hit
GCGTTCCAGGACGTTACCTTGGGAACGGTTCTCCGCATCACGCT9300.23316627262402315No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT9220.2311605412466122No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9030.22639692922526117No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG8990.2253940635365557No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8690.21787257087126466No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG8360.20959892893944446No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT8360.20959892893944446No Hit
TTCCATCGGCTGCTGCGGGCTTACCTGGGTCAGCTGCGGTGGCT8280.2075931975620335No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC8060.20207743627415337No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT7920.1985674063636842No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7780.19505737645321505No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT7740.19405451076450958No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC7650.19179806296492227No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG7490.18778660021010035No Hit
CGGGAAAGACAGATCTTCGTCAGAAGAAGAGCTGGATGCCGCAG7450.18678373452139488No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG7010.17575221194563465No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6950.17424791341257645No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG6870.1722421820351655No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6770.1697350178134018No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT6650.16672642074728536No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT6630.16622498790293264No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6440.1614613758815816No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6410.16070922661505252No Hit
GGACTGGTCCGGCACGTGCAGAACCGGCAGGTGCATGCGAGGCG6340.15895421165981793No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6320.15845277881546518No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6130.15368916679411418No Hit
GTCACCGTCCCACAGGTAGGTGAAGCGGATCTTTTTGCGGTCGC5990.15017913688364504No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT5940.14892555477276317No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5790.14516480844011764No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5560.13939833073006114No Hit
GCGCTGACGACGTTCCAGAACGTTGCCCTGGGATTTGTTTTCGA5520.13839546504135566No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA5390.13513615155306288No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG5340.13388256944218102No Hit
CGGGTCCTGGGACCACGGAGCCCACGGATGACGGTAGCACGGGT5270.13212755448694644No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5040.12636107677688996No Hit
GGACTGGCCACGGTGGCCACCATCGTAGCGTTCCCAGCCGCTCG5010.12560892751036085No Hit
CGGCGGCAGCGGCATCGGAGGGATGTTGTAGAAAACGGTCGGGG4970.12460606182165539No Hit
GCCACCATGGGCGCCTTTGCAACCGATACAAGACGGACGTTTCT4900.1228510468664208No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG4890.12260033044424444No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC4680.11733528557854069No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT4630.11608170346765884No Hit
CATGGACTCTTCGTGCAGCAGTACTGCAGTAACTTGAGGGCCCG4630.11608170346765884No Hit
CAGCAGAAATTCCTCTTCACCGGATTCCATCGGCGCGTGCTTGC4570.11457740493460063No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG4550.11407597209024789No Hit
CGGAGCCTGCGGTGGCGGCGGCTCTTGATAACCCTGATACGGCG4550.11407597209024789No Hit
CGGCGAAGCGACCGTTGAAAAAGGTGGATGCAACTGATTTAAAT4530.11357453924589515No Hit
CAGGTTGTGAGATGCACGGGTACGACGGTCTTCCGGGGTATCGT4500.11282238997936604No Hit
GCCGCTAGCGTCGTGACGATATTCCCACTGCTCGCCCTGTGCAG4410.11056594217977872No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA4350.1090616436467205No Hit
CTGCATCTGGTGCATAACGCGATGAGACGGCGCACGTGCCAGCA4300.10780806153583866No Hit
GTCGCCCGGCAGCGGTGCAGTCGGAGCCCACGGGGAGTTACCGT4120.10329516593666402No Hit
GTTGTAGAAAACGGTAGGGCTACGCGGTTCCGGGGAATCTGGGT4100.10279373309231127No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA4020.10078800171490032No Hit
CGTACTCCTGGCGACAGAGAAAATGGCGGTGACGTTATTTAAAT4020.10078800171490032No Hit
GACGTTCTCTTCTTCGTCGGAGTCCTTCAGGTGACGGTAACCGT4010.10053728529272395No Hit
CTGGACGGAAACAGAACGACGGCTACGTTTGGAGTGGCGGGTAC3990.10003585244837121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)