FastQCFastQC Report
Wed 31 May 2023
EGAF00007960069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398857
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT87022.181734305778763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37580.9421923145387945No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26420.6623927873899669No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA24070.60347442817852No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG18910.4741047543355137No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC18750.47009329158069185No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC16510.4139328130131852No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC14720.36905457344361514No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14590.36579525995532236No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14070.3527580060021511No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13800.3459886626033892No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC13700.3434814983816255No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT13400.33596000571633444No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG13070.32768636378451427No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12750.3196634382748704No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT12690.31815913974181226No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG12570.3151505426756958No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC11620.29133248256894073No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11420.2863181541254134No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA11280.2828081242149442No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT11200.2808023928375333No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11040.27679093008271133No Hit
CGTCAGCGTGACGCAGAAAACCGTAGCCAGG10080.2527221535537799No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9750.24444851162195974No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC9710.24344564593325427No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9680.24269349666672518No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9380.23517200400143412No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9040.22664764564743756No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8950.22439119784785022No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA8920.22363904858132114No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8700.218123287293441No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA8690.21787257087126466No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8400.21060179462814993No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8390.2103510782059736No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC8390.2103510782059736No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC8330.20884677967291537No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8210.20583818260679893No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC7970.19982098847456609No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7820.19606024214192053No Hit
GGTATGGATGAACCACCGTCTGGTTGGGAAC7760.19455594360886233No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA7660.19204877938709863No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC7340.18402585387745482No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA7190.1802651075448093No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6950.17424791341257645No Hit
TCTCGTACCAAACACCGTGACGGTTACCGTC6760.16948430139122542No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6430.16121065945940524No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6420.16095994303722888No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6350.1592049280819943No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA6300.15795134597111243No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC6230.15619633101587788No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6150.1541905996384669No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5970.1496777040392923No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5950.14917627119493954No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT5790.14516480844011764No Hit
GACGTAGAGAACAAATCTCAGGGTAACGTGC5760.14441265917358853No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5650.14165477852964847No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5630.14115334568529572No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5560.13939833073006114No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5510.1381447486191793No Hit
GGTCACCGTCTGAAACAAGCAGGTAACACTC5420.135888300819592No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5320.1333811365978283No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5070.12711322604341907No Hit
GCTACCCCAGAAAGCCAGGCCCCAACCGTCG5010.12560892751036085No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4970.12460606182165539No Hit
CTGCGTGTATTCGCTTGTTGCTGCGTTAAAC4950.12410462897730265No Hit
CAGCCTACTCCGTCCCCGCCGCGCATGCACC4850.12159746475553897No Hit
GGTTGGGGTCGTCGCTACGAACCAGCACCGA4800.12034388264465712No Hit
GGCGGTGATAACCACGGTCGCGGTCGTGGTC4710.1180874348450698No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT4650.11658313631201157No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT4570.11457740493460063No Hit
CGCGCATCTATCGGTTCTAAAAGCGTCTCTA4530.11357453924589515No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4220.1058023301584277No Hit
CGTGTTCGTGGTCACTACCGTGCTCCGTGGG4210.10555161373625134No Hit
ACCACCCTGCCGCCGACTCCGCTGCTGACCG4200.10530089731407496No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4190.10505018089189859No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC4150.10404731520319312No Hit
GGTCGTCTGGGTGTCGGCCCGCAGGTAACCG4130.10354588235884038No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT4100.10279373309231127No Hit
CGCCTGGACGCAGAACAGCGCGCCCAGCAGA4050.10154015098142943No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4030.10103871813707668No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)