FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007960071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426713
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT88072.0639164965679506No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC28420.6660214242359619No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA27210.6376651285524462No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21020.49260275641942003No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC20110.4712769472690075No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC18050.42300093974169994No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15620.36605399882356526No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC15620.36605399882356526No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14770.34613428697977333No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT14620.34261904371322177No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14530.3405098977532908No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC14480.3393381499977737No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT14190.3325420130157741No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG14030.3287924201981191No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG13860.32480847782936073No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13790.3231680309716367No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC13130.30770096059880997No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA12910.30254527047453444No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12690.29738958035025886No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT12520.29340563798150043No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11740.2751263729954325No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10750.2519257674361925No Hit
CGTCAGCGTGACGCAGAAAACCGTAGCCAGG10710.25098836923177875No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10670.250050971027365No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10440.244660931351986No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC10410.2439578826986757No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10030.2350525997567452No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG10010.2345839006545383No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9860.2310686573879868No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA9810.22989690963246961No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC9690.2270847150192284No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC9370.21958552938391848No Hit
GGTATGGATGAACCACCGTCTGGTTGGGAAC9260.21700768432178066No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9230.21630463566847039No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC9170.21489853836184977No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA9120.2137267906063326No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9050.21208634374860852No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8700.20388410945998836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8630.20224366260226428No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA8520.19966581754012647No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8400.1968536229268853No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA8300.19451012741585094No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7650.17927740659412766No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7510.17599651287867957No Hit
TCTCGTACCAAACACCGTGACGGTTACCGTC7350.17224692006102463No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA7230.1694347254477834No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC7190.16849732724336966No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC7180.16826297769226622No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7010.16427903532350785No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6780.15888899564812883No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT6710.15724854879040479No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6610.15490505327937046No Hit
GGTCACCGTCTGAAACAAGCAGGTAACACTC6540.1532646064216464No Hit
GACGTAGAGAACAAATCTCAGGGTAACGTGC6380.14951501360399144No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6100.14295322617309528No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6030.1413127793153712No Hit
CAGCCTACTCCGTCCCCGCCGCGCATGCACC5970.13990668200875062No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5940.1392036333554403No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5800.1359227396399922No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5780.13545404053778534No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5760.13498534143557847No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5670.13287619547564755No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5590.1310013990668201No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5580.13076704951571666No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5480.1284235540046823No Hit
GCTACCCCAGAAAGCCAGGCCCCAACCGTCG5410.12678310714695826No Hit
GGTTGGGGTCGTCGCTACGAACCAGCACCGA5330.12490831073813079No Hit
CTGCGTGTATTCGCTTGTTGCTGCGTTAAAC5210.12209611612488955No Hit
GGCGGTGATAACCACGGTCGCGGTCGTGGTC5210.12209611612488955No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT4870.11412823138737277No Hit
CGCGCATCTATCGGTTCTAAAAGCGTCTCTA4650.1089725412630972No Hit
GGTCGTCTGGGTGTCGGCCCGCAGGTAACCG4610.10803514305868346No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4500.10545729799654568No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4440.10405120068992509No Hit
CGTGTTCGTGGTCACTACCGTGCTCCGTGGG4440.10405120068992509No Hit
ACCACCCTGCCGCCGACTCCGCTGCTGACCG4400.10311380248551134No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA4340.10170770517889073No Hit
CGTGTTCGTGGCCACTATCGTGCGCCATGGG4290.10053595742337355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)