FastQCFastQC Report
Sun 21 May 2023
EGAF00007960073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405167
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT85882.119619811090242No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35470.8754414846223902No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25920.6397362075391135No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA25330.6251743108397277No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19660.48523201544054667No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC18440.4551209748079187No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC16890.4168651444959733No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC14940.36873684184546124No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14620.360838863974608No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14250.35170682706143397No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC14130.348745085359864No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT13990.34528972004136566No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13290.3280128934488742No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT13100.32332346908805504No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12770.3151786794087376No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG12740.31443824398334513No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG12700.31345099674948845No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC11610.2865485096268946No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA11540.28482082696764544No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11050.2727270483529014No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT10970.2707525538851881No Hit
CGTCAGCGTGACGCAGAAAACCGTAGCCAGG10120.2497735501657341No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9930.245084125804915No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9770.2411351368694884No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9620.2374329597425259No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9560.23595208889174094No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC9390.23175628814785013No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA9330.23027541729706513No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9270.22879454644628017No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9050.22336468666006853No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8990.22188381580928357No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8980.22163700400081943No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8970.22139019219235526No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC8950.22089656857542692No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8760.21620714421460782No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC8460.20880278996068288No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8230.2031261183660071No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA8150.20115162389829377No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8130.20065800028136546No Hit
GGTATGGATGAACCACCGTCTGGTTGGGAAC8040.19843669400518796No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC7890.19473451687822552No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA7820.19300683421897638No Hit
TCTCGTACCAAACACCGTGACGGTTACCGTC7280.1796789965619115No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC7150.17647044305187737No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6900.17030014784027328No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6850.16906608879795243No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC6380.15746593380013674No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6290.15524462752395926No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT6250.1542573802901026No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6160.15203607401392513No Hit
GGTCACCGTCTGAAACAAGCAGGTAACACTC6040.14907433231235515No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT6010.14833389688696266No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA6010.14833389688696266No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6000.1480870850784985No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5980.14759346146157015No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5950.1468530260361777No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5910.14586577880232102No Hit
GACGTAGAGAACAAATCTCAGGGTAACGTGC5800.14315084890921523No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5480.13525287103836195No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5460.13475924742143364No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG5370.13253794114525616No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5360.132291129336792No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5150.12710808135904453No Hit
GGTTGGGGTCGTCGCTACGAACCAGCACCGA5130.12661445774211622No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5020.12389952784901041No Hit
GCTACCCCAGAAAGCCAGGCCCCAACCGTCG5010.12365271604054624No Hit
CAGCCTACTCCGTCCCCGCCGCGCATGCACC4990.12315909242361792No Hit
CTGCGTGTATTCGCTTGTTGCTGCGTTAAAC4990.12315909242361792No Hit
GGCGGTGATAACCACGGTCGCGGTCGTGGTC4890.12069097433897627No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4490.11081850200040971No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA4480.11057169019194554No Hit
CGCGCATCTATCGGTTCTAAAAGCGTCTCTA4480.11057169019194554No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4370.10785676029883974No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT4270.10538864221419811No Hit
ACCACCCTGCCGCCGACTCCGCTGCTGACCG4220.10415458317187727No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT4190.10341414774648477No Hit
GGTCGTCTGGGTGTCGGCCCGCAGGTAACCG4170.10292052412955645No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA4160.10267371232109228No Hit
CGTGTTCGTGGTCACTACCGTGCTCCGTGGG4140.10218008870416395No Hit
GTAGGTTCTGAACCAATTTCTCCAGAGATCG4090.10094602966184314No Hit
GGTGGTGACAACCACGGTCGCGGTCGCGGTC4080.10069921785337897No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)