FastQCFastQC Report
Wed 8 May 2024
EGAF00007960074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007960074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499734
Total Bases21.9 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG28430.5689026562131053No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA27700.5542948848787554No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA26980.5398872200010406No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG20560.41141887484141565No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC15960.31936990478934796No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG15120.30256096243201386No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC13880.2777477618092825No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG13460.2693432906306155No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG12850.25713679677588475No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA12450.24913253851048758No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12360.24733158040077322No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA12340.24693136748750333No Hit
GGTATCGGAAGAGTCGTCATCCTCTTCTTCGGAGTCTTCTACCG12220.24453009000788417No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG12180.24372966418134445No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG11730.23472487363277264No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG11650.2331240219796932No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG11400.22812136056381996No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT11150.22311869914794671No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG10790.21591486670908927No Hit
GGTACGGTGAGTGGCACGGTCCTTCAGGTCGTAACGGGTGCTAG10730.21471422796927966No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA10700.21411390859937487No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG10560.21131241820648586No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG10060.20130709537473937No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10000.20010645663492976No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9980.19970624372165993No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9700.19410326293588187No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC9400.188100069236834No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG9360.1872996434102943No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA9210.18429804656077034No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG9190.18389783364750048No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG8190.1638871879840075No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8130.1626865492441979No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC8120.162486442787563No Hit
CAGACGTGGTTTGGTGGACGGTTTGTTAGTCTGGGTAGAAGTAG8100.16208622987429314No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT8060.16128580404775342No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7780.15568282326197536No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA7420.14847899082311788No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC7280.14567750043022887No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6790.13587228405511734No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6690.133871219488768No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6360.12726770641981533No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6270.125466748310101No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6160.12326557728711675No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6150.1230654708304818No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6090.12186483209067223No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5910.1182629158712435No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT5900.11806280941460856No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG5850.11706227713143393No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5780.11566153193498942No Hit
ACGCGGATCTTGACGGTTCGGCGGGTAGCCGTCGGAAGAGTCGG5580.11165940280229082No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5540.1108589769757511No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG5290.10585631555987786No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5280.10565620910324293No Hit
AACAGAGGACAGACGGTCACGGGTCTCACGAGAACCGCGTTCAC5250.10505588973333813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)